Penicillin-resistant mechanisms in Pseudomonas aeruginosa: effects of penicillin G and carbenicillin on transpeptidase and C -alanine carboxypeptidase activities.

Antimicrobial agents and chemotherapy ·Vol. 6 ·No. 6 ·1974-12-00 ·Pages 672-5

Suginaka H, Ichikawa A, Kotani S

Abstract

A membrane fraction from Pseudomonas aeruginosa KM 338 was shown to catalyze in vitro peptidoglycan synthesis from uridine 5'-diphosphate-N-acetylmuramyl-l-alanyl-d- glutamyl-meso-diaminopimelyl-d-alanyl-d-alanine and uridine 5'-diphosphate-N-acetylglucosamine. Synthesized peptidoglycan was partially cross-linked by transpeptidation, which was accompanied by the release of d-alanine. This reaction was strongly inhibited by 25 and 50 mug of penicillin G and carbenicillin per ml respectively, whereas the intact cells were relatively resistant to penicillins (minimal inhibitory concentration of penicillin G and carbenicillin, 30 and 0.125 mg/ml, respectively). Soluble d-alanine carboxypeptidase present in P. aeruginosa KM 338 was studied as well, which was found almost completely inhibited by penicillin G and carbenicillin (10 mug/ml).

MeSH Terms

Acyltransferases/metabolism Alanine Carbenicillin/pharmacology Carbon Radioisotopes Carboxypeptidases/metabolism Depression, Chemical Penicillin G/pharmacology Penicillin Resistance/drug effects Peptides Peptidoglycan/biosynthesis Pseudomonas aeruginosa/enzymology,metabolism

Chemicals

Carbon Radioisotopes Peptides Peptidoglycan Acyltransferases Carboxypeptidases Carbenicillin Alanine Penicillin G

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Suginaka H

Ichikawa A

Kotani S

References (19)

19 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.6.6.672

Published

1974-12-00

Pages

672-5

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC444717

Subset

External Links

PubMed source DOI: 10.1128/AAC.6.6.672 PMC Full Text View journal details

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Penicillin-resistant mechanisms in Pseudomonas aeruginosa: effects of penicillin G and carbenicillin on transpeptidase and C -alanine carboxypeptidase activities.

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