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PMID: 4217590 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Detecting the enterotoxigenicity of Staphylococcus aureus strains.

Applied microbiology ·Vol. 28 ·No. 6 ·1974-12-00 ·Pages 946-50

Robbins R, Gould S, Bergdoll M

Abstract

An optimal sensitivity plate method for examining large number of staphylococcal strains for production of the known enterotoxins (A-E) is presented. Small volumes of relatively concentrated enterotoxin are produced by the semi-solid agar, cellophane-over-agar, or sac culture techniques. Detection of the enterotoxin in the supernatant fluid is accomplished with the optimal sensitivity plate method. In this method small plastic petri dishes (50 mm) were used for a modified Ouchterlony of high sensitivity.

MeSH Terms
Agar Cellophane Enterotoxins/analysis,biosynthesis Immunodiffusion Staphylococcus/growth & development,metabolism
Chemicals
Enterotoxins Agar Cellophane
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Robbins R
Gould S
Bergdoll M
References (10)
10 references, click to expand
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    Appl Microbiol. 1970 Apr;19(4):698-9 PMID: 4986729
  2. Serological detection of enterotoxin from food-poisoning strains of Staphylococcus aureus.
    J Med Microbiol. 1971 Feb;4(1):19-30 PMID: 4994125
  3. PRODUCTION OF LARGE QUANTITIES OF ENTEROTOXIN B AND OTHER STAPHYLOCOCCAL TOXINS ON SOLID MEDIA.
    Acta Pathol Microbiol Scand. 1965;63:299-305 PMID: 14295449
  4. [Cultivation of staphylococci for the collection of larger amounts of enterotoxins].
    Zentralbl Bakteriol Orig A. 1972 Apr;219(4):426-34 PMID: 4145476
  5. DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD.
    Appl Microbiol. 1965 Mar;13:181-9 PMID: 14325876
  6. Detectionn of staphylococcal enterotoxin by the gel diffusion test.
    Can J Public Health. 1972 Jan-Feb;63(1):58-66 PMID: 4622041
  7. Serological identification of enterotoxigenic staphylococci from cheese.
    Appl Microbiol. 1967 Nov;15(6):1382-7 PMID: 16349749
  8. Identification of a new enterotoxin as enterotoxin C.
    J Bacteriol. 1965 Nov;90(5):1481-5 PMID: 4954560
  9. CULTURE MEDIUM FOR THE PRODUCTION OF STAPHYLOCOCCAL ENTEROTOXIN A.
    J Bacteriol. 1963 Jul;86:18-23 PMID: 14051816
  10. Production of enterotoxin a.
    Appl Microbiol. 1966 Nov;14(6):966-72 PMID: 16349703
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
DOI
10.1128/am.28.6.946-950.1974
Published
1974-12-00
Pages
946-50
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC186861
Subset
IM
External Links
PubMed source DOI: 10.1128/am.28.6.946-950.1974 PMC Full Text
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