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PMID: 4218230 Published · ppublish English Journal Article

Activation of inactive nitrogenase by acid-treated component I.

Journal of bacteriology ·Vol. 120 ·No. 2 ·1974-11-00 ·Pages 697-701

Nagatani HH, Shah VK, Brill WJ

Abstract

When Azotobacter vinelandii was derepressed for nitrogenase synthesis in a N-free medium containing tungstate instead of molybdate, an inactive component I was synthesized. Although this inactive component I could be activated in vivo upon addition of molybdate to the medium, it could not be activated in vitro when molybdate was added to the extracts. Activation occurred, however, when an acid-treated component I was added to extracts of cells derepressed in medium containing tungstate. Acid treatment completely abolished component I activity. Mutant strains UW45 and UW10 were unable to fix N(2). Both strains synthesized normal levels of component II but produced inactive component I. Acid-treated component I activated inactive component I in extracts of mutant strain UW45 but not mutant strain UW10. This activating factor could be obtained from N(2)-fixing Klebsiella pneumoniae, Clostridium pasteurianum, and Rhodospirillum rubrum.

MeSH Terms
Azotobacter/enzymology,immunology Bacterial Proteins/pharmacology Clostridium Culture Media Enzyme Activation/drug effects Indicators and Reagents Klebsiella Klebsiella pneumoniae Molybdenum/metabolism Mutation Nitrogen Fixation Nitrogenase/metabolism Rhodospirillum rubrum Tungsten/metabolism
Chemicals
Bacterial Proteins Culture Media Indicators and Reagents Molybdenum Nitrogenase Tungsten
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nagatani H H
Shah V K
Brill W J
References (25)
25 references, click to expand
  1. The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.
    Proc Natl Acad Sci U S A. 1966 Sep;56(3):979-86 PMID: 5230193
  2. Nitrogenase V. The effect of Mo, W and V on the synthesis of nitrogenase components in Azotobacter vinelandii.
    Biochim Biophys Acta. 1974 Aug 7;362(1):160-6 PMID: 4422774
  3. In vitro assembly of Neurospora assimilatory nitrate reductase from protein subunits of a Neurospora mutant and the xanthine oxidizing or aldehyde oxidase systems of higher animals.
    Proc Natl Acad Sci U S A. 1970 Jul;66(3):1016-23 PMID: 4393266
  4. In vitro formation of assimilatory nitrate reductase: presence of the constitutive component in bacteria.
    Biochem Biophys Res Commun. 1973 Jun 19;52(4):1450-6 PMID: 4268718
  5. The role of tungsten in the inhibition of nitrate reductase activity in spinach (spinacea oleracea L.) leaves.
    Biochem Biophys Res Commun. 1971 Aug 6;44(3):702-10 PMID: 4107835
  6. Tungsten incorporation into Azotobacter vinelandii nitrogenase.
    FEBS Lett. 1973 Feb 1;29(3):219-221 PMID: 11946917
  7. Reversible cyanide inhibition of spinach (Spinacea oleracea L.) nitrate reductase and non-exchangeability in vitro of protein bound molybdenum and tungsten.
    FEBS Lett. 1971 Oct 15;18(1):19-22 PMID: 11946072
  8. Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins.
    Biochem J. 1972 Jul;128(3):655-75 PMID: 4344006
  9. Restoration of reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase activity of a Neurospora mutant by extracts of various chlorate-resistant mutants of Escherichia coli.
    J Bacteriol. 1972 Oct;112(1):388-91 PMID: 4404057
  10. Purification and characterization of the molybdenum-iron protein component of nitrogenase from soybean nodule bacteroids.
    J Biol Chem. 1974 Jan 25;249(2):500-8 PMID: 4855615
  11. Nitrogenase. IV. Simple method of purification to homogeneity of nitrogenase components from Azotobacter vinelandii.
    Biochim Biophys Acta. 1973 May 30;305(2):445-54 PMID: 4354875
  12. In vitro formation of nitrate reductase using extracts of the nitrate reductase mutant of Neurospora crassa, nit-1, and Rhodospirillum rubrum.
    J Bacteriol. 1973 Nov;116(2):600-9 PMID: 4270447
  13. Invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3242-6 PMID: 4399835
  14. Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra.
    Biochim Biophys Acta. 1973 Jan 18;292(1):246-55 PMID: 4350172
  15. N,O-Diacetylneuraminic acid and N-acetylneuraminic acid in Escherichia coli.
    Nature. 1959 Aug 1;184(Suppl 6):381-2 PMID: 13815135
  16. Tungstate antagonism of molybdate in Aspergillus niger.
    Proc Soc Exp Biol Med. 1956 Jul;92 (3):509-11 PMID: 13359449
  17. Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii.
    Biochim Biophys Acta. 1972 Feb 28;256(2):498-511 PMID: 5016550
  18. Reduction of N2 by complementary functioning of two components from nitrogen-fixing bacteria.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):537-41 PMID: 5245987
  19. Competition between Free and Combined Nitrogen in Nutrition of Azotobacter.
    Proc Natl Acad Sci U S A. 1943 Sep;29(9):289-94 PMID: 16578092
  20. Tungstate as competitive inhibitor of molybdate in nitrate assimilation and in N2 fixation by Azotobacter.
    Biochim Biophys Acta. 1957 Feb;23 (2):433-5 PMID: 13412744
  21. Purification and some properties of molybdoferredoxin, a component of nitrogenase from Clostridium pasteurianum.
    Biochemistry. 1971 May 25;10(11):2066-72 PMID: 4327398
  22. Evidence for common genetic determinants of nitrogenase and nitrate reductase in Rhizobium meliloti.
    Nat New Biol. 1973 Dec 5;246(153):153-4 PMID: 4519145
  23. EFFECT OF TUNGSTATE ON THE UPTAKE AND FUNCTION OF MOLYBDATE IN AZOTOBACTER AGILIS.
    J Bacteriol. 1961 Jul;82(1):130-4 PMID: 16561910
  24. Isolation by crystallization of the Mo-Fe protein of Azotobacter nitrogenase.
    Biochem Biophys Res Commun. 1970 Apr 8;39(1):90-9 PMID: 5438303
  25. Tungstate as an antagonist of molybdate in Azotobacter vinelandii.
    Arch Biochem Biophys. 1957 Aug;70(2):585-90 PMID: 13459414
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.120.2.697-701.1974
Published
1974-11-00
Pages
697-701
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245829
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.120.2.697-701.1974 PMC Full Text View journal details
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