The localization of adenosine triphosphatases in morphologically characterized subcellular fractions of guinea-pig brain.

The Biochemical journal ·Vol. 96 ·No. 2 ·1965-08-00 ·Pages 404-12

Hosie RJ

Abstract

1. The distribution of adenosine triphosphatase was studied in morphologically characterized subcellular fractions of guinea-pig brain. The conditions of homogenization were selected so as to favour the survival of nerve endings as organized structures. 2. A fraction consisting mainly of the external membranes of nerve endings was rich in a ouabain-sensitive Na(+)-K(+)-stimulated adenosine triphosphatase which closely resembled that present in the classical microsomal fraction studied by other workers, but which showed a higher specific activity. 3. A dinitrophenol-stimulated adenosine triphosphatase was located in the nerve-ending mitochondria. 4. The synaptic-vesicle fraction contained a small amount of adenosine triphosphatase that differed in its response to several ions and other compounds from the membrane, myelin and mitochondrial fractions, indicating freedom from contamination by these elements.

MeSH Terms

Acetylcholine Adenosine Triphosphatases/metabolism Animals Brain/enzymology Calcium Chelating Agents Chemistry Techniques, Analytical Dinitrophenols Enzymes Guinea Pigs In Vitro Techniques Ions Magnesium Microscopy, Electron Microsomes/enzymology Norepinephrine Ouabain Potassium/pharmacology Sodium/pharmacology Subcellular Fractions Ultrasonics

Chemicals

Chelating Agents Dinitrophenols Enzymes Ions Ouabain Sodium Adenosine Triphosphatases Magnesium Acetylcholine Potassium Calcium Norepinephrine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Hosie R J

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27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0960404

Published

1965-08-00

Pages

404-12

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1207054

Subset

External Links

PubMed source DOI: 10.1042/bj0960404 PMC Full Text View journal details

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The localization of adenosine triphosphatases in morphologically characterized subcellular fractions of guinea-pig brain.

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