Citrate and the regulation of adipose-tissue phosphofructokinase.

The Biochemical journal ·Vol. 100 ·No. 2 ·1966-08-00 ·Pages 420-3

Denton RM, Randle PJ

Abstract

1. Methods are described for the extraction, partial purification and assay of phosphofructokinase in rat epididymal adipose tissue. 2. The enzyme was inhibited by ATP at concentrations above 20mum and by citrate; the enzyme was activated by ADP, AMP, 3',5'-(cyclic)-AMP, phosphate and sulphate. 3. The enzyme lost activity on incubation at 25 degrees or during chromatography on DEAE-cellulose unless fluoride at a concentration of 4mm was present. 4. The significance of these results in relation to the role of glycolysis and citrate in lipogenesis is discussed.

MeSH Terms

Adenine Nucleotides/pharmacology Adenosine Triphosphate/pharmacology Adipose Tissue/enzymology Animals Chemical Phenomena Chemistry Chromatography Citrates/pharmacology Fluorides/pharmacology Glycolysis Lipids/biosynthesis Male Phosphates/pharmacology Phosphofructokinase-1/metabolism Rats Spectrophotometry Sulfates/pharmacology

Chemicals

Adenine Nucleotides Citrates Lipids Phosphates Sulfates Adenosine Triphosphate Phosphofructokinase-1 Fluorides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Denton R M

Randle P J

References (13)

13 references, click to expand

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Adenosine 3',5'-monophosphate in biological materials. II. The measurement of adenosine 3',5'-monophosphate in tissues and the role of the cyclic nucleotide in the lipolytic response of fat to epinephrine.
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Citrate cleavage in adipose tissue.
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The role of phosphofructokinase in metabolic regulation.
Adv Enzyme Regul. 1964;2:265-74 PMID: 4222062
STUDIES ON HEART PHOSPHOFRUCTOKINASE. ACTIVE AND INACTIVE FORMS OF THE ENZYME.
J Biol Chem. 1965 May;240:2165-72 PMID: 14299642
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Nature. 1963 Oct 12;200:169-70 PMID: 14073034
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[ON THE BIOSYNTHESIS OF FATTY ACIDS. V. ACETYL-COA CARBOXYLASE FROM RAT LIVER AND ITS ACTIVATION BY CITRIC ACID].
Biochem Z. 1964 Aug 11;340:263-89 PMID: 14317958
PROPERTIES OF PHOSPHOFRUCTOKINASE FROM RAT LIVER AND THEIR RELATION TO THE CONTROL OF GLYCOLYSIS AND GLUCONEOGENESIS.
Biochem J. 1965 Jun;95:868-75 PMID: 14342527
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1000420

Published

1966-08-00

Pages

420-3

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1265151

Subset

External Links

PubMed source DOI: 10.1042/bj1000420 PMC Full Text View journal details

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Citrate and the regulation of adipose-tissue phosphofructokinase.

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