Investigation of the component reactions of oxidative sterol demethylation. Evidence against participation of cytochrome P-450.

Animals Bile Acids and Salts Chromatography, Gel Chromatography, Ion Exchange Cyanides/pharmacology Depression, Chemical Heme/metabolism Liver/enzymology,metabolism Male Microsomes/enzymology,metabolism Mixed Function Oxygenases/isolation & purification,metabolism Niacinamide/pharmacology Oxidoreductases Pigments, Biological/metabolism Rats Sterols Surface-Active Agents Transferases/antagonists & inhibitors,metabolism Trypsin

Bile Acids and Salts Cyanides Pigments, Biological Sterols Surface-Active Agents Niacinamide Heme Mixed Function Oxygenases Oxidoreductases Transferases Trypsin