Latent Salinity Stress Detection in Opuntia ficus-indica Using Hyperspectral Imaging and a 3D-CNN Framework.

Salinity stress remains a major bottleneck for agriculture in arid regions. While Opuntia ficus-indica is known for its resilience, its young cladodes maintain a misleadingly healthy visual appearance and stable biomass even under heavy saline pressure, making traditional vegetation indices and standard statistics unreliable for early diagnosis. The objective of this study was to develop a non-destructive phenotyping framework for the early detection of latent salinity stress in young Opuntia cladodes. Controlled experiments were conducted using hyperspectral data cubes (400-1000 nm) acquired from plants exposed to six distinct salinity levels ranging from 2 to 21 dS m-1. Our methodology integrates these high-dimensional spatial-spectral data with a tailor-made 3D Convolutional Neural Network (3D-CNN). Seven physiological vegetation indices-NDVI, PRI, WI, PSRI, MCARI, SIPI, and NDRE were extracted to track sub-clinical shifts and processed as a volumetric depth dimension within the network to preserve spatial-spectral integrity. The optimized 3D-CNN framework achieved a validation accuracy of 99.7% and a weighted F1-score of 99.1%, delivering 100% precision at critical stress thresholds (13 and 21 dS m-1). Spatial confidence maps (Softmax > 0.95) further confirmed the high reliability of the diagnostic output. Requiring a training duration of approximately 8 s, this framework provides a robust basis for precision early-warning irrigation systems to sustain Opuntia cultivation in challenging environments.