The complete mitochondrial genome of the Boulenophrys xianjuensis (Anura: Megophryidae).

Boulenophrys xianjuensis is a member of the family Megophryidae and was first described in 2020 in Xianju County, Zhejiang Province, China. In this study, the complete mitochondrial genome of B. xianjuensis was sequenced, assembled, and annotated using next-generation sequencing technology. The assembled mitogenome is a circular molecule 17,602 base pairs (bp) in length, with a GC content of 41.40%, and contains 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes, and a single control region (D-loop). The majority of PCGs are encoded on the heavy strand (H-strand), whereas one PCG (ND6) and eight tRNA genes (trnE(uuc), trnS(tga), trnY(gua), trnC(gca), trnN(guu), trnA(ugc), trnQ(uug), and trnP(ugg)) are encoded on the light strand (L-strand). Phylogenetic analysis placed B. xianjuensis within a well-supported clade comprising other Boulenophrys species, with the genus Boulenophrys forming a sister group to Atympanophrys. This new newly characterized mitogenome provides valuable molecular data for understanding the mitochondrial genome of B. xianjuensis and contributes significantly to the clarification of the phylogenetic relationships within the genus Boulenophrys.