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PMID: 424305 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A correlation between nucleosome spacer region susceptibility to DNase I and histone acetylation.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 561-74

Nelson D, Perry ME, Chalkley R

Abstract

Hepatoma tissue culture (HTC) cell nuclei were digested with either DNase I or micrococcal nuclease and the nucleohistone digestion products fractionated by gel electrophoresis or exclusion chromatography. Under appropriate conditions, gel electrophoresis demonstrates that for both nucleases, only cleavages within the nucleosome spacer regions and not within the nucleosome core lead to freely migrating nucleohistone particles. These particles consist of nucleosome cores, nucleosomes and nucleosome oligomers. Following DNase I digestion and fractionation by exclusion chromatography, analysis of the histones indicates a direct relationship between increased spacer region susceptibility to nuclease and increased nucleosomal histone acetylation. Evidently digestion sites outside the regions of DNA protected by core histones can reflect the degree of acetylation of core histones. Such a relationship is not found when micrococcal nuclease is used to digest the samples.

MeSH Terms
Acetylation Animals Cell Line Chromatin/analysis DNA, Neoplasm Deoxyribonucleases Histones Liver Neoplasms, Experimental/analysis Micrococcal Nuclease Rats
Chemicals
Chromatin DNA, Neoplasm Histones Deoxyribonucleases Micrococcal Nuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nelson D
Perry M E
Chalkley R
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.2.561
Published
1979-02-00
Pages
561-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327713
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.2.561 PMC Full Text View journal details
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