Binding of deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase to poxvirus.

Journal of virology ·Vol. 9 ·No. 1 ·1972-01-00 ·Pages 70-4

Tan KB, McAuslan BR

Abstract

Poxvirus has a deoxyribonucleic acid polymerase activity that remains associated with the virus despite repeated centrifugation through sucrose gradients. Highly purified poxvirus preparation can adsorb deoxyribonucleic acid polymerase from cytoplasmic extracts of cells containing such an activity. These results indicate that caution must be used in assuming that an enzyme associated with a purified virus is necessarily an integral part of the virion.

MeSH Terms

Adsorption Animals Cell Line Centrifugation, Density Gradient Cricetinae Culture Techniques DNA DNA Nucleotidyltransferases/analysis,metabolism DNA Viruses/enzymology,growth & development,isolation & purification Floxuridine/pharmacology HeLa Cells Hot Temperature Humans Kidney Nucleic Acid Denaturation Poxviridae/enzymology Salmonidae Sucrose Surface-Active Agents Thymine Nucleotides/metabolism Tritium Vaccinia virus/enzymology,growth & development,isolation & purification

Chemicals

Surface-Active Agents Thymine Nucleotides Floxuridine Tritium Sucrose DNA DNA Nucleotidyltransferases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tan K B

McAuslan B R

References (14)

14 references, click to expand

DNA polymerase activity associated with purified Kilham rat virus.
Nat New Biol. 1971 Jun 9;231(23):174-6 PMID: 5281195
DNA ligase and exonuclease activities in virions of rous sarcoma virus.
Nat New Biol. 1971 Apr 21;230(16):232-5 PMID: 4324864
Vaccinia virus structural polypeptide derived from a high-molecular-weight precursor: formation and integration into virus particles.
J Virol. 1970 Dec;6(6):717-26 PMID: 4250367
Effect of rifampicin on poxvirus protein synthesis.
J Virol. 1970 Sep;6(3):326-32 PMID: 5480409
DNA-directed DNA polymerase activity in oncogenic RNA viruses.
Nature. 1970 Sep 5;227(5262):1029-31 PMID: 4317810
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization.
Biochem Biophys Res Commun. 1971 Aug 20;44(4):879-85 PMID: 5166448
Two deoxyribonuclease activities within purified vaccinia virus.
Proc Natl Acad Sci U S A. 1969 Jul;63(3):820-7 PMID: 5259766
RNA polymerase activity in purified reoviruses.
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462-9 PMID: 4179180
Localization of nucleotide phosphohydrolase activity within vaccinia.
Proc Natl Acad Sci U S A. 1968 Jul;60(3):845-52 PMID: 4299268
Poxvirus DNA-dependent RNA polymerase.
Proc Natl Acad Sci U S A. 1967 Jul;58(1):134-41 PMID: 5231593
Studies on "early" enzymes in HeLa cells infected with vaccinia virus.
Virology. 1965 Sep;27(1):80-93 PMID: 4157610
Proteins of polyhedral cytoplasmic deoxyviruses. I. The structural polypeptides of FV 3 .
Virology. 1971 Jul;45(1):200-7 PMID: 5165250
RNA-dependent DNA polymerase in virions of RNA tumour viruses.
Nature. 1970 Jun 27;226(5252):1209-11 PMID: 4316300

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.9.1.70-74.1972

Published

1972-01-00

Pages

70-4

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356263

Subset

External Links

PubMed source DOI: 10.1128/JVI.9.1.70-74.1972 PMC Full Text View journal details

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Binding of deoxyribonucleic acid-dependent deoxyribonucleic acid polymerase to poxvirus.

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