Therapeutic Magnetic Resonance (TMR) in Regenerative Medicine: In Vitro Study to Support Future Clinical Applications.

This in vitro study investigates the effects of therapeutic magnetic resonance (TMR®), a novel biophysical stimulation technology, on mesenchymal stromal cells (MSCs) to support its potential application in regenerative medicine. The role of pulsed electromagnetic fields in bone healing is already established. We evaluated whether TMR® influences MSC proliferation, differentiation, and immunomodulatory properties in vitro. Bone marrow-derived MSCs (BM-MSCs) and adipose-derived MSCs (AD-MSCs) were cultured with or without TMR® exposure and assessed through flow cytometry, karyotype analysis, senescence assays, proliferation tests, gene expression analysis, and osteogenic differentiation assays. TMR® did not alter MSC phenotype, proliferation, senescence, or genomic stability, confirming its safety profile. Notably, treated MSCs showed enhanced osteogenic differentiation, with increased early expression of key osteogenic markers (RUNX2, ALP, and COL1A1) and greater collagen deposition compared to untreated controls. TMR® reduced peripheral blood mononuclear cell proliferation and MSC ROS production, suggesting anti-inflammatory and antioxidative effects. Overall, TMR® appears to be a safe, non-invasive stimulus able to promote osteogenic differentiation, supporting its potential clinical application in bone regeneration. Further in vitro studies and clinical trials are needed to confirm these findings.