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PMID: 4264701 Published · ppublish English Journal Article

Isolation and characterization of rabbit kidney brush borders.

The Biochemical journal ·Vol. 128 ·No. 5 ·1972-08-00 ·Pages 1319-28

Quirk SJ, Robinson GB

Abstract

1. Brush borders were isolated from rabbit kidney-cortex homogenates by rate-zonal centrifugation through a sucrose density gradient in a B-XIV zonal rotor, followed by differential centrifugation. 2. The method of preparation gave brush borders of high purity with a reasonable yield. The morphological appearance supported the evidence from enzymic and chemical investigations, that the brush borders were only slightly contaminated with endoplasmic reticulum, mitochondria, lysosomes and nuclei. 3. The molar ratio of cholesterol to phospholipid lay within the range found in other plasma membranes, but the carbohydrate content was double that found in liver plasma membranes. 4. Alkaline phosphatase, maltase, trehalase and aminopeptidase were major enzymic constituents of the brush borders, and had an approximately equal yield and enrichment, but none of these enzymes fulfilled the criteria for marker enzymes. 5. Mg(2+)-dependent and Na(+),K(+)-dependent adenosine triphosphatases, although found in brush borders, had low yields and low enrichments.

MeSH Terms
Adenosine Triphosphatases/analysis Alkaline Phosphatase/analysis Aminopeptidases/analysis Animals Cell Fractionation Cell Nucleus Centrifugation, Density Gradient Centrifugation, Zonal Cholesterol/blood Endoplasmic Reticulum Epithelium/enzymology Glucosidases/analysis Kidney/cytology Kidney Cortex/cytology Lysosomes Magnesium Microscopy, Electron Mitochondria Phospholipids/blood Potassium Rabbits Sodium Trehalase/analysis
Chemicals
Phospholipids Cholesterol Sodium Alkaline Phosphatase Glucosidases Trehalase Aminopeptidases Adenosine Triphosphatases Magnesium Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Quirk S J
Robinson G B
References (51)
51 references, click to expand
  1. The enteric surface coat on cat intestinal microvilli.
    J Cell Biol. 1965 Dec;27(3):475-91 PMID: 4223093
  2. [Structural and activity identity of leucine aminopeptidase from pig kidney and bovine lens, and comparison with particulate aminopeptidase from pig kidney].
    Hoppe Seylers Z Physiol Chem. 1967 Jun;348(6):689-704 PMID: 5601648
  3. Lipid composition of rat liver plasma membranes.
    J Biol Chem. 1969 Oct 25;244(20):5528-36 PMID: 4310600
  4. The isolation of a brush border membrane fraction from rat kidney.
    J Biol Chem. 1970 Nov 25;245(22):6106-12 PMID: 4320796
  5. Rat intestinal microvillus membranes. Purification and biochemical characterization.
    Biochem J. 1968 Jan;106(2):381-90 PMID: 4295331
  6. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  7. Lipid composition of the isolated rat intestinal microvillus membrane.
    Biochem J. 1968 Aug;109(1):51-9 PMID: 5669848
  8. Presence of a "cell coat" rich in carbohydrate at the surface of cells in the rat.
    Anat Rec. 1966 Jan;154(1):41-71 PMID: 4162458
  9. Enzymology of kidney tissue.
    Curr Probl Clin Biochem. 1968;2:119-45 PMID: 4359230
  10. SPECIFIC ASSAYS OF SOME PHOSPHATASES IN SUBCELLULAR FRACTIONS OF SMALL INTESTINAL MUCOSA.
    Nature. 1965 Feb 20;205:799-800 PMID: 14292314
  11. A direct lead method for the electron microscopic visualization of alkaline phosphatase activity.
    J Histochem Cytochem. 1966 May;14(5):429-31 PMID: 5961999
  12. The distribution of some hydrolases in glomeruli and tubular fragments prepared from rat kidney by zonal centrifugation.
    Biochem J. 1971 May;122(5):641-5 PMID: 4331586
  13. Structural and functional organization of the brush border of intestinal epithelial cells. 3. Enzymic activities and chemical composition of various fractions of tris-disrupted brush borders.
    Biochim Biophys Acta. 1967 Jul 3;135(3):475-82 PMID: 4227786
  14. Distribution of (Na+-K+)-stimulated ATPase activity in rat intestinal mucosa.
    Biochim Biophys Acta. 1969 Apr;173(3):456-68 PMID: 4305976
  15. Histochemical localization of renal trehalase: demonstration of a tubular site.
    Science. 1968 Aug 9;161(3841):571-2 PMID: 5663295
  16. The colorimetric determination of phosphorus.
    Biochem J. 1932;26(2):292-7 PMID: 16744823
  17. [Arylamidase activity of glomeruli isolated from rat kidneys].
    Klin Wochenschr. 1969 Nov 1;47(21):1178-9 PMID: 5382011
  18. The determination of carbohydrate in biological materials by gas-liquid chromatography.
    Methods Biochem Anal. 1971;19:229-344 PMID: 4935452
  19. The enzymatic hydrolysis of amino acid beta-naphthylamides. II. Partial purification and properties of a particle-bound cobalt-activated rat kidney aminopeptidase.
    J Histochem Cytochem. 1966 May;14(5):401-13 PMID: 5961997
  20. Some features of the lipid composition of rat liver surface and cytoplasmic membranes.
    Chem Phys Lipids. 1968 Feb;2(1):144-6 PMID: 5698575
  21. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  22. The relation of structural and catalytic protein in the mitochondrial electron transfer chain.
    Arch Biochem Biophys. 1966 Jul;115(1):215-25 PMID: 4290771
  23. Brush border particulates of renal tissue.
    Science. 1968 Nov 29;162(3857):1009-11 PMID: 4301646
  24. Studies on plasma membranes. 13. Co 2+ -activated aminopeptidase(s) in the globular units locally coating rat-liver plasma membranes.
    Biochim Biophys Acta. 1971 Aug 13;241(2):273-89 PMID: 5003613
  25. An evaluation of aminopeptidase specificity with seven chromogenic substrates.
    Arch Biochem Biophys. 1962 May;97:223-31 PMID: 14477774
  26. Carbohydrates in cell surfaces.
    Int Rev Cytol. 1970;29:77-125 PMID: 5509867
  27. Isolation of brush border fragments from homogenates of rat and rabbit kidney cortex.
    Exp Cell Res. 1968 Jul;51(1):123-40 PMID: 4873819
  28. Effect of fixation on the alkaline phosphatase activity of mouse proximal convuluted tubule.
    J Histochem Cytochem. 1967 Jul;15(7):413-6 PMID: 4860984
  29. [On the demonstration and substrate specificity of a kidney particle aminopeptidase distinguishable from leucine aminopeptidase].
    Hoppe Seylers Z Physiol Chem. 1967 Jun;348(6):680-8 PMID: 5601647
  30. Isolation and biochemical characterization of brush borders from rabbit kidney.
    J Cell Biol. 1970 Dec;47(3):637-45 PMID: 4250612
  31. The digestive function of the epithelium of the small intestine. I. An intracellular locus of disaccharide and sugar phosphate ester hydrolysis.
    Biochim Biophys Acta. 1961 Sep 16;52:281-93 PMID: 14473958
  32. Multiple molecular forms of leucine aminopeptidase in man.
    Acta Genet Stat Med. 1966;16(3):223-30 PMID: 5953709
  33. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.
    Biochem J. 1961 Sep;80:649-54 PMID: 13734134
  34. Membraneous ultrastructural demonstration of aminopeptidase and gamma-glutamyl transpeptidase activities with a new diazonium salt that yields a lipophobic, osmiophilic azo dye.
    J Histochem Cytochem. 1970 Aug;18(8):542-51 PMID: 5449981
  35. Characterization of plasma membrane proteins in mammalian kidney. I. Preparation of a membrane fraction and separation of the protein.
    J Biol Chem. 1969 Jul 10;244(13):3561-9 PMID: 4240115
  36. [Isolation and enzymatic characterization of a brushborder fraction from rat kidney].
    Pflugers Arch. 1969;308(1):1-15 PMID: 4305867
  37. Biochemical analysis of the pinocytotic process. I. Isolation and chemical composition of the lysosomal and the plasma membrane of the rat liver cell.
    Hoppe Seylers Z Physiol Chem. 1970 Oct;351(10):1191-9 PMID: 5479418
  38. The demonstration of alkaline phosphatase in the electron microscope.
    J Biophys Biochem Cytol. 1960 Apr;7:387-90 PMID: 14423173
  39. Glycoproteins.
    Annu Rev Biochem. 1970;39:599-638 PMID: 4921697
  40. Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat.
    J Biol Chem. 1971 Oct 25;246(20):6339-46 PMID: 4108384
  41. (Na+K+)-activated ATPase in kidney cell membranes of normal and methyprednisolone-treated rats.
    Biochim Biophys Acta. 1968 Jun 11;150(4):563-71 PMID: 4298265
  42. (1-14C)glucosamine incorporation by subcellular fractions of small intestinal mucosa. Identification by precursor labeling of three functionally distinct glycoprotein classes.
    J Biol Chem. 1970 Jul 25;245(14):3584-92 PMID: 5470824
  43. Assay of intestinal disaccharidases.
    Anal Biochem. 1968 Jan;22(1):99-107 PMID: 5636962
  44. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  45. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  46. Action of neuraminidase on human kidney alkaline phosphatase.
    Nature. 1966 Feb 19;209(5025):805-6 PMID: 5922152
  47. Quantitative histochemistry of the nephron.
    Science. 1958 Jun 6;127(3310):1342-3 PMID: 13555890
  48. Electron microscope studies on the surface coat of the nephron.
    J Cell Biol. 1969 Mar;40(3):585-601 PMID: 5765757
  49. Plasma membranes: phospholipid and sterol content.
    Science. 1966 Jan 14;151(3707):210-1 PMID: 5907912
  50. Isolation of rat liver plasma membranes. Use of nucleotide pyrophosphatase and phosphodiesterase I as marker enzymes.
    J Cell Biol. 1970 Dec;47(3):604-18 PMID: 5497542
  51. Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat.
    J Cell Biol. 1967 Jan;32(1):27-53 PMID: 10976200
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1281319
Published
1972-08-00
Pages
1319-28
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1174020
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1281319 PMC Full Text View journal details
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