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PMID: 4268690 Published · ppublish English Journal Article

R-factor-mediated resistance to tetracycline in Proteus mirabilis.

Journal of bacteriology ·Vol. 115 ·No. 1 ·1973-07-00 ·Pages 235-42

Franklin TJ, Rownd R

Abstract

The expression of R-factor-mediated resistance to tetracycline has been compared in Proteus mirabilis and Escherichia coli. Resistance to a range of concentrations of tetracycline was significantly lower in P. mirabilis than in E. coli in both induced and repressed states. Indirect evidence showed that conditions which result in a marked increase in the level of resistance of P. mirabilis harboring the R factor NR1 to chloramphenicol, streptomycin, and spectinomycin due to an amplification in the number of copies of r-determinants per cell do not detectably increase the level of resistance to tetracycline. Tetracycline resistance was inducible in early stationary-phase P. mirabilis NR1 although not after 5 h in this state. Double isotope labeling of control and tetracycline-induced P. mirabilis NR1 in early stationary phase revealed isotopic enrichment of certain peaks in extracts from induced cells subjected to polyacrylamide gel electrophoresis.

MeSH Terms
Acetyltransferases/metabolism Bacterial Proteins/biosynthesis Carbon Isotopes Chloramphenicol/pharmacology Drug Resistance, Microbial Electrophoresis, Polyacrylamide Gel Escherichia coli/drug effects Genetics, Microbial Leucine/metabolism Proteus/drug effects Proteus mirabilis/drug effects,enzymology,metabolism Species Specificity Spectinomycin/pharmacology Streptomycin/pharmacology Sulfonamides/pharmacology Tetracycline/pharmacology Tritium
Chemicals
Bacterial Proteins Carbon Isotopes Sulfonamides Tritium Chloramphenicol Spectinomycin Acetyltransferases Tetracycline Leucine Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Franklin T J
Rownd R
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18 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.115.1.235-242.1973
Published
1973-07-00
Pages
235-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246235
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.115.1.235-242.1973 PMC Full Text View journal details
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