In vitro induction of specific immune responses in the absence of serum: requirement for nonspecific T or B cell mitogens.

Animals Antibody Formation Antibody-Producing Cells Antigens B-Lymphocytes/immunology Blood Concanavalin A Culture Media DNA/biosynthesis Dinitrophenols Erythrocytes/immunology Escherichia coli/immunology Hemolytic Plaque Technique Immunization, Secondary Lipopolysaccharides Lymphocyte Activation Lymphocyte Culture Test, Mixed Mice Polysaccharides, Bacterial Sheep/immunology Spleen/cytology T-Lymphocytes/immunology Thymidine/metabolism Tritium

Antigens Culture Media Dinitrophenols Lipopolysaccharides Polysaccharides, Bacterial Tritium Concanavalin A DNA Thymidine