Use of neomycin in the isolation of mutants blocked in energy conservation in Escherichia coli.

Journal of bacteriology ·Vol. 111 ·No. 1 ·1972-07-00 ·Pages 287-9

Kanner BI, Gutnick DL

Abstract

A neomycin-resistant mutant of Escherichia coli K-12 unable to grow on Krebs cycle intermediates has been isolated. The mutant retained its respiratory capacity, but lacked membrane Mg(2+)-Ca(2+)-stimulated adenosine triphosphatase activity (EC 3.6.1.3).

MeSH Terms

Adenosine Triphosphatases/metabolism Adenosine Triphosphate/biosynthesis Aerobiosis Anaerobiosis Calcium/pharmacology Cell Membrane/enzymology Cell-Free System Culture Media Drug Resistance, Microbial Escherichia coli/drug effects,enzymology,growth & development,isolation & purification,metabolism Glucose/metabolism Ketoglutaric Acids/metabolism Magnesium/pharmacology Malates/metabolism Mutation Neomycin/pharmacology Nitrates/metabolism Oxygen Consumption Succinates/metabolism

Chemicals

Culture Media Ketoglutaric Acids Malates Nitrates Succinates Adenosine Triphosphate Adenosine Triphosphatases Neomycin Magnesium Glucose Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kanner B I

Gutnick D L

References (12)

12 references, click to expand

The function of ubiquinone in Escherichia coli.
Biochem J. 1970 Apr;117(3):551-62 PMID: 4192611
Mutants of Escherichia coli requiring methionine or vitamin B12.
J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles.
Virology. 1960 Nov;12:348-90 PMID: 13764402
The growth of micro-organisms in relation to their energy supply.
J Gen Microbiol. 1960 Dec;23:457-69 PMID: 13687855
OXIDATIVE PHOSPHORYLATION COUPLED WITH NITRATE RESPIRATION. II. PHOSPHORYLATION COUPLED WITH ANAEROBIC NITRATE REDUCTION IN A CELL-FREE EXTRACT OF ESCHERICHIA COLI.
J Biochem. 1964 Feb;55:131-5 PMID: 14135451
Hemin-deficient mutants of Escherichia coli K-12.
J Bacteriol. 1968 Aug;96(2):570-2 PMID: 4877132
Oxidative phosphorylation in yeast. V. Phosphorylation efficiencies in growing cells determined from molar growth yields.
Biochim Biophys Acta. 1969 May;180(1):9-17 PMID: 5787273
Hemin-deficient mutants of Salmonella typhimurium.
J Bacteriol. 1970 May;102(2):531-6 PMID: 4911544
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Membrane adenosine triphosphatase of Escherichia coli: activation by calcium ion and inhibition by monovalent cations.
J Bacteriol. 1969 Nov;100(2):914-22 PMID: 4242923
Membrane Mg-(Ca)-Activated Adenosine Triphosphatase of Escherichia coli: Characterization in the Membrane-Bound and Solubilized States.
J Bacteriol. 1970 Dec;104(3):1203-12 PMID: 16559094
Oxidative phosphorylation in Escherichia coli K12. Mutations affecting magnesium ion- or calcium ion-stimulated adenosine triphosphatase.
Biochem J. 1971 Aug;124(1):75-81 PMID: 4256722

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.111.1.287-289.1972

Published

1972-07-00

Pages

287-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC251270

Subset

External Links

PubMed source DOI: 10.1128/jb.111.1.287-289.1972 PMC Full Text View journal details

Analysis Services

Use of neomycin in the isolation of mutants blocked in energy conservation in Escherichia coli.

Contact

WeChat Official Account