A rat-tissue aminotransferase acting on L-tyrosine O-sulphate.

The Biochemical journal ·Vol. 98 ·No. 1 ·1966-01-00 ·Pages 168-72

Rose FA, Flanagan TH, John RA

Abstract

1. Rat tissues have been shown to possess an aminotransferase that is active towards l-tyrosine O-sulphate and dependent on 2-oxoglutarate and pyridoxal phosphate. 2. Kidney, liver and pancreas have the greatest activity and the enzyme is localized mainly in the mitochondrial fraction in the liver and kidney cell. 3. The enzyme was shown to be distinct from l-tyrosine-2-oxoglutarate aminotransferase but its true identity was not established. 4. A procedure for the assay of the enzyme in crude tissue preparations was developed.

MeSH Terms

Animals Electron Transport Complex IV/metabolism Hydrocortisone/pharmacology In Vitro Techniques Ketoglutaric Acids Kidney/enzymology L-Lactate Dehydrogenase/metabolism Liver/enzymology Pancreas/enzymology Pyridoxal Phosphate Rats Subcellular Fractions/metabolism Sulfates/metabolism Transaminases/metabolism Tyrosine/metabolism

Chemicals

Ketoglutaric Acids Sulfates Tyrosine Pyridoxal Phosphate L-Lactate Dehydrogenase Electron Transport Complex IV Transaminases Hydrocortisone

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rose F A

Flanagan T H

John R A

References (15)

15 references, click to expand

Tyrosine-O-sulfate as a constituent of normal human urine.
J Biol Chem. 1955 Dec;217(2):703-8 PMID: 13271432
Intracellular distribution of tyrosine-alpha-ketoglutarate transaminase and 4-C-14-hydrocortisone activities during induction.
J Biol Chem. 1963 Jan;238:302-5 PMID: 13931011
AMINO-ACID SEQUENCE INVESTIGATIONS OF FIBRINOPEPTIDES FROM VARIOUS MAMMALS: EVOLUTIONARY IMPLICATIONS.
Nature. 1964 Apr 11;202:147-52 PMID: 14156289
A microspectrophotometric method for the determination of cytochrome oxidase.
J Biol Chem. 1951 Apr;189(2):665-70 PMID: 14832284
3:5 Diiodo-L-tyrosine-alpha-ketoglutarate transamination by extracts of rat kidney mitochondria.
Endocrinology. 1963 Feb;72:296-304 PMID: 13937464
Effect of a pyrimido-pyrimidine derivative on non-specific enzymic stimulation in vivo.
Biochim Biophys Acta. 1962 Jan 29;56:593-4 PMID: 14465931
Induction of tyrosine-alpha-ketoglutarate transaminase in rat liver. III. Immunochemical analysis.
J Biol Chem. 1962 May;237:1610-4 PMID: 14455204
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.
Biochem J. 1955 Aug;60(4):604-17 PMID: 13249955
The enzymic properties of rat-liver nuclei. 1. Estimation of the extent to which contaminant material contributes to the activity observed in the nuclear fraction.
Biochem J. 1956 Oct;64(2):361-8 PMID: 13363849
The assay of aromatic amino acid transaminations and keto acid oxidation by the enol borate-tautomerase method.
J Biol Chem. 1958 Sep;233(3):668-73 PMID: 13575433
Observations on the metabolism of tyrosine O[35S]-sulphate in the rat.
Biochem J. 1961 May;79:209-13 PMID: 13723348
Studies on l-tyrosine O-sulphate. 2. Identification of p-hydroxyphenylpyruvic acid [S]sulphate as a metabolite of l-tyrosine O[S]-sulphate in the rat.
Biochem J. 1963 Jun;87(3):545-8 PMID: 16749016
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Studies on l-tyrosine O-sulphate. 1. Preparation and properties of potassium p-hydroxyphenylpyruvic acid [S]sulphate.
Biochem J. 1963 Jun;87(3):541-5 PMID: 16749015
Induction of tyrosine-alpha-ketoglutarate transaminase in rat liver. I. Hormonal nature.
J Biol Chem. 1961 Oct;236:2699-702 PMID: 14455202

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0980168

Published

1966-01-00

Pages

168-72

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1264810

Subset

External Links

PubMed source DOI: 10.1042/bj0980168 PMC Full Text View journal details

Analysis Services

A rat-tissue aminotransferase acting on L-tyrosine O-sulphate.

Contact

WeChat Official Account