Factors controlling amino acid incorporation by ribosomes from krebs II mouse ascites-tumour cells.

The Biochemical journal ·Vol. 98 ·No. 3 ·1966-03-00 ·Pages 826-35

Kerr IM, Cohen N, Work TS

Abstract

1. A ribosome-cell sap system capable of supporting the incorporation of (14)C-labelled amino acids into protein has been prepared from Krebs II mouse ascites-tumour cells. The requirements of this system for optimum activity and response to added messenger RNA have been investigated. One such system has been obtained for which amino acid incorporation is almost wholly dependent on the addition of suitable messenger RNA. 2. Ribosomes of widely different but predictable activities in the cell-free system have been prepared from Krebs cells pretreated in a variety of ways. The factors in the pretreatment of the cells responsible for these differences have been investigated. 3. The structural and functional properties of these different ribosome preparations and their response to exogenous messenger RNA have been examined and are discussed in the light of modern concepts of the control of protein synthesis.

MeSH Terms

Adenosine Triphosphate/metabolism Amino Acids/metabolism Animals Carcinoma, Krebs 2/metabolism Dactinomycin/pharmacology Encephalomyocarditis virus In Vitro Techniques Mice Neoplasm Proteins/biosynthesis RNA, Messenger/metabolism RNA, Viral Ribosomes/metabolism

Chemicals

Amino Acids Neoplasm Proteins RNA, Messenger RNA, Viral Dactinomycin Adenosine Triphosphate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kerr I M

Cohen N

Work T S

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24 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0980826

Published

1966-03-00

Pages

826-35

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1264925

Subset

External Links

PubMed source DOI: 10.1042/bj0980826 PMC Full Text View journal details

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Factors controlling amino acid incorporation by ribosomes from krebs II mouse ascites-tumour cells.

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