Production of high-titer antibody in serum and ascitic fluid of hamsters for a variety of virus-induced tumor antigens.

Applied microbiology ·Vol. 15 ·No. 3 ·1967-05-00 ·Pages 657-60

Gilden RV, Beddow TG, Huebner RJ

Abstract

Methods not involving the use of viable tumors were developed for production of high-titer antibodies. The methods involved use of tumor homogenates. Homogenates from all tumor lines tested contained the characteristic tumor antigen. Attempts to isolate virus failed, and virus antigens could not be demonstrated. Immunization with tumor homogenates, in Freund's complete adjuvant, resulted in high antibody levels. None of the animals so immunized developed tumors.

MeSH Terms

Animals Antibodies, Neoplasm/analysis,blood Antibodies, Viral/analysis,blood Antigens, Viral, Tumor/immunology Ascites/immunology Complement Fixation Tests Cricetinae Humans Neoplasms, Experimental/immunology Oncogenic Viruses/immunology Polyomavirus/immunology Sarcoma, Avian/immunology Simian virus 40/immunology

Chemicals

Antibodies, Neoplasm Antibodies, Viral Antigens, Viral, Tumor

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gilden R V

Beddow T G

Huebner R J

References (12)

12 references, click to expand

Hamster ascitic fluids containing complement-fixing antibody against virus-induced tumor antigens.
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Proc Natl Acad Sci U S A. 1965 Aug;54(2):381-8 PMID: 4285931
Application of a microtechnique to viral serological investigations.
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SPECIFIC ADENOVIRUS COMPLEMENT-FIXING ANTIGENS IN VIRUS-FREE HAMSTER AND RAT TUMORS.
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SPECIFIC COMPLEMENT-FIXING VIRAL ANTIGENS IN HAMSTER AND GUINEA PIG TUMORS INDUCED BY THE SCHMIDT-RUPPIN STRAIN OF AVIAN SARCOMA.
Proc Natl Acad Sci U S A. 1964 May;51:742-50 PMID: 14174396
AN AVIAN LEUCOSIS GROUP-SPECIFIC COMPLEMENT FIXATION REACTION. APPLICATION FOR THE DETECTION AND ASSAY OF NON-CYTOPATHOGENIC LEUCOSIS VIRUSES.
Virology. 1964 Jul;23:313-21 PMID: 14194126
EXPRESSION OF POLYOMA-INDUCED CELLULAR ANTIGEN(S) IN HYBRID CELLS.
Nature. 1964 Aug 1;203:495-6 PMID: 14202368
IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES.
J Exp Med. 1964 Oct 1;120:577-88 PMID: 14212121
SPECIFIC COMPLEMENT-FIXING TUMOR ANTIGENS IN HUMAN CELLS MORPHOLOGICALLY TRANSFORMED BY SV40 VIRUS.
Proc Natl Acad Sci U S A. 1964 Dec;52:1316-8 PMID: 14243502
SPECIFIC COMPLEMENT-FIXING ANTIGENS IN POLYOMA TUMORS AND TRANSFORMED CELLS.
Virology. 1965 Jan;25:55-61 PMID: 14277085
PRODUCTION OF "TUMOR-SPECIFIC" ANTIGENS BY ONCOGENIC VIRUSES DURING ACUTE CYTOLYTIC INFECTIONS.
Proc Natl Acad Sci U S A. 1965 Jan;53:12-9 PMID: 14283190
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.15.3.657-660.1967

Published

1967-05-00

Pages

657-60

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC546999

Subset

External Links

PubMed source DOI: 10.1128/am.15.3.657-660.1967 PMC Full Text

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Production of high-titer antibody in serum and ascitic fluid of hamsters for a variety of virus-induced tumor antigens.

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