Isolation of deoxyribonucleic acid from mammalian tissues.

The Biochemical journal ·Vol. 104 ·No. 1 ·1967-07-00 ·Pages 254-7

Kirby KS, Cook EA

Abstract

1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides remained in solution when DNA was precipitated with 2-butoxyethanol in the presence of 0.5m-sodium chloride and 1.5m-sodium benzoate. 4. Ribosomal RNA was removed by precipitation in the presence of 3m-sodium chloride at 0 degrees , when DNA remained soluble.

MeSH Terms

Animals Carcinoma, Hepatocellular Centrifugation DNA/analysis DNA, Neoplasm/analysis Hot Temperature In Vitro Techniques Liver/analysis Liver Neoplasms Nucleic Acid Denaturation RNA/analysis Rats Ribosomes/analysis Sarcoma, Experimental Spleen/analysis

Chemicals

DNA, Neoplasm RNA DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kirby K S

Cook E A

References (11)

11 references, click to expand

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Biochem J. 1967 Jul;104(1):258-62 PMID: 4962318
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Br J Cancer. 1960 Mar;14:147-50 PMID: 14409278
The preparation of deoxyribonucleic acids by the p-aminosalicylate-phenol method.
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Br J Cancer. 1964 Dec;18:792-800 PMID: 14264946
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1040254

Published

1967-07-00

Pages

254-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1270569

Subset

External Links

PubMed source DOI: 10.1042/bj1040254 PMC Full Text View journal details

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Isolation of deoxyribonucleic acid from mammalian tissues.

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