Avirulent Clostridium perfringens strains obtained by euflavine treatment.

Journal of bacteriology ·Vol. 94 ·No. 5 ·1967-11-00 ·Pages 1437-42

Paquette G, Fredette V

Abstract

Clostridium perfringens was incubated in the presence of euflavine (EU); the resistant mutants which were thus isolated had highly reduced capacity to release alpha-toxin. This fact was confirmed by lecithinase determinations and by immunoelectrophoresis. Injected into guinea pigs and into 6- to 7-day-old chicks, these mutants were completely avirulent. A study of their properties indicated biochemical differences between wild types and mutants.

MeSH Terms

Acridines/metabolism,pharmacology Animals Carbohydrate Metabolism Caseins/metabolism Chickens Clostridium Infections Clostridium perfringens/drug effects,enzymology,pathogenicity Female Guinea Pigs Hemolysis Hydrogen-Ion Concentration Immunoelectrophoresis Male Mice Mutation/drug effects Phospholipases/biosynthesis,metabolism Toxins, Biological/biosynthesis Virulence

Chemicals

Acridines Caseins Toxins, Biological Phospholipases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Paquette G

Fredette V

References (8)

8 references, click to expand

Use of the Waring blendor for reducing the viscosity of fluid cultures of the perfringens bacillus.
Can J Microbiol. 1962 Apr;8:275-7 PMID: 13894835
The isolation of the alpha-toxin of Clostridium welchii type A by zone electrophoresis in vertical columns.
Biochem J. 1961 Sep;80(3):578-84 PMID: 16748926
Acriflavine as an effective agent for eliminating F-factor in Escherichia coli K-12.
Nature. 1957 Sep 28;180(4587):655-6 PMID: 13477247
The biochemistry of the gas gangrene toxins: Estimation of the alpha toxin of Cl. welchii, type A.
Biochem J. 1941 Nov;35(10-11):1246-56 PMID: 16747410
The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins.
Biochem J. 1941 Sep;35(8-9):884-902 PMID: 16747456
[Effect of acriflavine on yeasts. VIII. Determination of the active component and study of euflavine].
Ann Inst Pasteur (Paris). 1951 Oct;81(4):452-68 PMID: 14894912
Genetic studies on microbial cross resistance to toxic agents. I. Cross resistance of Escherichia coli to fifteen antibiotics.
J Bacteriol. 1952 Oct;64(4):489-99 PMID: 12999676
The biochemistry of the gas gangrene toxins: Partial purification of the toxins of Cl. welchii, type A. Separation of alpha and theta toxins.
Biochem J. 1941 Nov;35(10-11):1257-69 PMID: 16747411

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.94.5.1437-1442.1967

Published

1967-11-00

Pages

1437-42

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC276844

Subset

External Links

PubMed source DOI: 10.1128/jb.94.5.1437-1442.1967 PMC Full Text View journal details

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Avirulent Clostridium perfringens strains obtained by euflavine treatment.

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