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PMID: 4300506 Published · ppublish English Journal Article

The lactose synthetase particles of lactating bovine mammary gland. Preparation of particles with intact lactose synthetase.

The Biochemical journal ·Vol. 109 ·No. 2 ·1968-09-00 ·Pages 169-76

Coffey RG, Reithel FJ

Abstract

1. The particulate form of lactating bovine mammary lactose synthetase activity is shown to be more highly organized than previously reported. 2. A novel method of shattering frozen mammary tissue with effective cell disruption is described. 3. The apparent subcellular distribution of lactose synthetase was shown to reflect the method of homogenization. 4. After mild homogenization particles associated with a high content of intact lactose synthetase activity sedimented in the lysosome size range between 5x10(4) and 3x10(5)g-min. 5. Lactose synthetase was dissociated and solubilized by VirTis homogenization and ultrasonic treatment. The activities and behaviour of UDP-galactose hydrolase, succinate dehydrogenase, beta-glucuronidase and phosphodiesterase I were compared. 6. Inhibition of UDP-galactose hydrolase by UTP and alpha-lactalbumin was observed.

MeSH Terms
Albumins Animals Cattle Centrifugation Female Freezing Glucosyltransferases/analysis Glucuronidase/metabolism In Vitro Techniques Lactation Ligases/analysis Lysosomes/enzymology Mammary Glands, Animal/enzymology Methods Phosphoric Monoester Hydrolases/metabolism Pregnancy Succinate Dehydrogenase/metabolism Tissue Extracts Ultrasonics Uracil Nucleotides
Chemicals
Albumins Tissue Extracts Uracil Nucleotides Succinate Dehydrogenase Glucosyltransferases Phosphoric Monoester Hydrolases Glucuronidase Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coffey R G
Reithel F J
References (18)
18 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1090169
Published
1968-09-00
Pages
169-76
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186771
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1090169 PMC Full Text View journal details
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