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PMID: 430595 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Physical characterization of the superhelical DNA genome of an enveloped mycoplasmavirus.

Journal of virology ·Vol. 29 ·No. 1 ·1979-01-00 ·Pages 374-80

Nowak JA, Maniloff J

Abstract

Mycoplasmavirus MVL2 is a nonlytic enveloped virion containing DNA. This DNA has been shown to be a double-stranded circular superhelical molecule of 11.8 kilobase pairs (7.8 X 10(6) daltons). The superhelix density is greater than that of phi X174 RFI but less than that of PM2 phage DNA. A physical map of the MVL2 genome has been obtained using restriction endonucleases.

MeSH Terms
Acholeplasma laidlawii/genetics Bacteriophages/analysis DNA Restriction Enzymes DNA, Superhelical/analysis DNA, Viral/analysis Genes, Viral Nucleic Acid Conformation
Chemicals
DNA, Superhelical DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nowak J A
Maniloff J
References (20)
20 references, click to expand
  1. Structure and reactions of closed duplex DNA.
    Annu Rev Biophys Bioeng. 1978;7:287-313 PMID: 208457
  2. Growth of an enveloped mycoplasmavirus and establishment of a carrier state.
    J Virol. 1977 May;22(2):308-14 PMID: 405508
  3. Viruses of mycoplasmas and spiroplasmas.
    Adv Virus Res. 1977;21:343-80 PMID: 324253
  4. Characterization of mycoplasmavirus MVL2 DNA.
    J Virol. 1979 Jan;29(1):381-4 PMID: 430596
  5. Nucleotide sequence of bacteriophage phi X174 DNA.
    Nature. 1977 Feb 24;265(5596):687-95 PMID: 870828
  6. Host cell and ultraviolet reactivation of ultraviolet-irradiated Mycoplasmaviruses.
    J Bacteriol. 1977 Mar;129(3):1424-7 PMID: 845120
  7. Restriction endonucleases.
    CRC Crit Rev Biochem. 1976 Nov;4(2):123-64 PMID: 795607
  8. Infection of Acholeplasma laidlawii by MVL51 virus.
    Virology. 1973 Sep;55(1):118-26 PMID: 4125688
  9. Mycoplasmatales virus-laidlawii 2, a new virus isolated from Acholeplasma laidlawii.
    J Gen Virol. 1971 Jul;12(1):65-7 PMID: 4106810
  10. Sensitivity of superhelical DNA to a single-strand specific endonuclease.
    Biochim Biophys Acta. 1973 Apr 21;308(7):68-78 PMID: 4269186
  11. A simple electrophoretic method for the determination of superhelix density of closed circular DNAs and for observation of their superhelix density heterogeneity.
    Anal Biochem. 1976 May 7;72:95-103 PMID: 182041
  12. Dye titrations of covalently closed supercoiled DNA analyzed by agarose gel electrophoresis.
    Biochem Biophys Res Commun. 1976 Mar 22;69(2):446-54 PMID: 178312
  13. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.
    J Mol Biol. 1974 Nov 15;89(4):783-801 PMID: 4449133
  14. Further studies on the morphology and composition of Mycoplasmatales virus-laidlawii 2.
    J Gen Virol. 1973 Feb;18(2):127-33 PMID: 4735309
  15. The gel electrophoresis of DNA.
    Biochim Biophys Acta. 1972 May 10;269(2):192-200 PMID: 5063906
  16. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
    Virology. 1970 Mar;40(3):734-44 PMID: 4908735
  17. Ethidium binding affinity of circular lambda deoxyribonucleic acid determined fluorometrically.
    J Biol Chem. 1975 Feb 10;250(3):1061-70 PMID: 1089648
  18. dna, single stranded/*metab.
    Virology. 1976 Sep;73(2):561-7 PMID: 960577
  19. Site of cleavage of superhelical phiX174 replicative form DNA by the single strand-specific Neurospora crassa endonuclease.
    J Biol Chem. 1976 Jan 25;251(2):530-5 PMID: 942717
  20. DNA of bacteriophage PM2: a closed circular double-stranded molecule.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8 PMID: 5260915
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1979-01-00
Pages
374-80
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353134
Subset
IM
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