Ferrous-activated nicotinamide adenine dinucleotide-linked dehydrogenase from a mutant of Escherichia coli capable of growth on 1, 2-propanediol.

Journal of bacteriology ·Vol. 98 ·No. 1 ·1969-04-00 ·Pages 87-95

Sridhara S, Wu TT, Chused TM, Lin EC

Abstract

A nicotinamide adenine dinucleotide-linked dehydrogenase has been partially purified from a mutant of Escherichia coli K-12 able to grow on l-1,2-propanediol as carbon and energy source. This enzyme catalyzes the dehydrogenation at carbon 1 of l-1,2-propanediol, glycerol, 1,3-propanediol, ethylene glycol, and ethyl alcohol. The purified protein requires added ferrous or managanous ions. The V(max) and the apparent K(m) for a given substrate vary with the particular metal used.

MeSH Terms

Centrifugation, Density Gradient Chromatography Escherichia coli/enzymology,metabolism Ethanol/metabolism Glycerol/metabolism Glycols/metabolism Iron/pharmacology Kinetics Manganese/pharmacology Mutation NAD Oxidoreductases/isolation & purification,metabolism Propylene Glycols/metabolism Spectrophotometry

Chemicals

Glycols Propylene Glycols NAD Ethanol Manganese Iron Oxidoreductases Glycerol

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Sridhara S

Wu T T

Chused T M

Lin E C

References (34)

34 references, click to expand

EVOLUTION OF A CATABOLIC PATHWAY IN BACTERIA.
Science. 1964 Dec 4;146(3649):1313-5 PMID: 14207460
A FUMARATE REDUCTASE IN ESCHERICHIA COLI DISTINCT FROM SUCCINATE DEHYDROGENASE.
J Biol Chem. 1963 Nov;238:3770-4 PMID: 14109218
"Hydroxylamine kinase" and pyruvic kinase.
J Biol Chem. 1960 Jul;235:1944-7 PMID: 14412832
Mutants of Escherichia coli constitutive for alkaline phosphatase.
J Bacteriol. 1961 May;81:835-6 PMID: 13777588
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.
J Biol Chem. 1961 May;236:1372-9 PMID: 13767412
Mutants of Aerobacter aerogenes capable of utilizing xylitol as a novel carbon.
J Bacteriol. 1968 Aug;96(2):447-56 PMID: 5674056
A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.
Biochim Biophys Acta. 1960 Mar 11;38:470-83 PMID: 13826559
A comparison of some vicglycol dehydrogenase systems found in Aerobacter aerogenes.
Biochim Biophys Acta. 1960 Feb 26;38:272-83 PMID: 14413633
CONTROL OF ETHANOL DEHYDROGENASE LEVELS IN AEROBACTER AEROGENES.
J Bacteriol. 1961 Jun;81(6):852-7 PMID: 16561903
[Sexuality of bacteria].
Boll Ist Sieroter Milan. 1950 Sep-Oct;29(9-10):281-9 PMID: 14801088
ZINC, A COMPONENT OF YEAST ALCOHOL DEHYDROGENASE.
Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):327-38 PMID: 16589675
Mutation of bacteria at high levels of survival by ethyl methane sulphonate.
Nature. 1959 Dec 5;184:1780-2 PMID: 14418494
The stereochemistry of the conversion of D and L 1,2-propanediols to propionaldehyde.
J Biol Chem. 1966 Jul 10;241(13):3028-35 PMID: 4287906
Metallocarboxypeptidases: stability constants and enzymatic characteristics.
J Biol Chem. 1961 Aug;236:2244-9 PMID: 13694603
The activation of glycerol dehydrogenase from Aerobacter aerogenes by monovalent cations.
J Biol Chem. 1960 Jun;235:1820-3 PMID: 14417009
A method for isolating constitutive mutants for carbohydrate-catabolizing enzymes.
Biochim Biophys Acta. 1962 Jul 2;60:422-4 PMID: 14465346
Growth of Aerobacter aerogenes on D-arabinose and L-xylose.
J Bacteriol. 1965 Oct;90(4):1157-8 PMID: 5847804
HUMAN LIVER--ALCOHOL DEHYDROGENASE. KINETIC AND PHYSICOCHEMICAL PROPERTIES.
Biochemistry. 1964 Nov;3:1775-82 PMID: 14235347
ENZYME SYSTEMS IN THE MYCOBACTERIA. XV. INITIAL STEPS IN THE METABOLISM OF GLYCEROL.
J Bacteriol. 1963 Jul;86:30-7 PMID: 14051819
The hydrolysis of purine and pyrimidine nucleoside triphosphates by myosin.
J Biol Chem. 1956 Mar;219(1):95-101 PMID: 13295260
Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.
Biochim Biophys Acta. 1960 Mar 11;38:460-9 PMID: 13838951
Proline and hydroxproline in leaves.
Nature. 1952 Oct 4;170(4327):579-80 PMID: 13002373
THE ORIENTATION AND EXTENT OF GENE TRANSFER IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1956 Sep;42(9):619-24 PMID: 16589917
THE CONTROL OF DISSIMILATION OF GLYCEROL AND L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI.
J Biol Chem. 1964 Sep;239:3106-8 PMID: 14217903
FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE.
Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):344-54 PMID: 16589677
Influence of cobalt and cadmium on the peptidase and esterase activities of carboxypeptidase B.
Biochim Biophys Acta. 1961 Mar 18;48:139-47 PMID: 13700531
FERMENTATION OF GLUCOSE BY SUSPENSIONS OF ESCHERICHIA COLI.
J Bacteriol. 1949 Feb;57(2):147-58 PMID: 16561659
Zinc in horse liver alcohol dehvdrogenase.
J Biol Chem. 1957 Mar;225(1):185-95 PMID: 13416229
A new chemical mutagen for bacteria, 1-methyl-3-nitro-1-nitrosoguanidine.
Biochem Biophys Res Commun. 1960 Dec;3:575-7 PMID: 13766074
Studies on the biosynthesis of L-rhammose.
J Biol Chem. 1958 Aug;233(2):287-91 PMID: 13563488
PATHWAYS OF L-ARABITOL AND XYLITOL METABOLISM IN AEROBACTER AEROGENES.
J Biol Chem. 1964 Jul;239:2110-5 PMID: 14209935
Dissimilation of glucose at controlled pH values by pigmented and non-pigmented strains of Escherichia coli.
J Bacteriol. 1956 Oct;72(4):497-9 PMID: 13366953
A simplified photometric analysis for serine and formaldehyde.
J Biol Chem. 1954 Apr;207(2):709-16 PMID: 13163057
A basis for utlization of unnatural pentoses and pentitols by Aerobacter aerogenes.
Proc Natl Acad Sci U S A. 1965 Aug;54(2):572-9 PMID: 5217444

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.98.1.87-95.1969

Published

1969-04-00

Pages

87-95

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC249908

Subset

External Links

PubMed source DOI: 10.1128/jb.98.1.87-95.1969 PMC Full Text View journal details

Analysis Services

Ferrous-activated nicotinamide adenine dinucleotide-linked dehydrogenase from a mutant of Escherichia coli capable of growth on 1, 2-propanediol.

Contact

WeChat Official Account