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PMID: 4307872 Published · ppublish English Journal Article

The role of individual spleen cells in the interferon response of the intact mouse.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 62 ·No. 4 ·1969-04-00 ·Pages 1038-45

Osborn JE, Walker DL

Abstract

A method is described for the detection of interferon production by individual spleen cells of mice after intravenous virus infection. Although mouse spleen plays a major role in the total interferon response to Newcastle disease virus, the number of spleen cells participating is a small fraction of the total, suggesting that interferon formation is to some extent a specialized cell function. Monolayers of mouse embryo cells, after brief contact with agar-suspended spleen cells from interferon-producing mice, have roughly circular foci of cells remaining after removal of agar and vesicular stomatitis virus challenge. The numbers of such foci correlate directly with the number of spleen cells, concentration of inducing virus in the inoculum, duration of contact of cells with mouse-embryo indicator monolayers, time after interferon induction, and serum interferon titer. With this technique, evolution of the interferon-forming cell response in spleens of virus-infected mice has been studied.

MeSH Terms
Animals Arboviruses Cell Line Culture Media Culture Techniques Embryo, Mammalian Encephalomyocarditis virus Interferons/biosynthesis,blood L Cells Methods Mice Newcastle disease virus Puromycin/pharmacology Spleen/immunology Vesicular stomatitis Indiana virus
Chemicals
Culture Media Puromycin Interferons
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Osborn J E
Walker D L
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.62.4.1038
Published
1969-04-00
Pages
1038-45
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223610
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.62.4.1038 PMC Full Text View journal details
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