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PMID: 4311797 Published · ppublish English Journal Article

In vitro transformation by adenovirus-simiam virus 40 hybrid viruses. IV. Properties of clones isolated from cell lines transformed by adenovirus 2-simiam virus 40 and adenovirus 12-simiam virus 40 transcapsidant hybird viruses.

Journal of virology ·Vol. 4 ·No. 5 ·1969-11-00 ·Pages 694-703

Black PH, Berman LD, Dixon CB

Abstract

Clones were isolated from hamster cells transformed by the adenovirus 2-SV40 and adenovirus 12-SV40 transcapsidant hybrid viruses. The clones were characterized with respect to their cytomorphology, virus and antigen content, and the histomorphology of tumors induced by transplantation of the clonal sublines to hamsters. Three different cellular and colonial morphologies were observed. Clones with an SV40 morphology gave rise to tumors predominantly with an SV40 histology, whereas clones with an adenovirus morphology produced typical adenovirus tumors upon transplantation of the transformed cells. Clones which had features of both SV40 and adenovirus transformed cells gave rise to "intermediate" and adenovirus tumors. The results indicate that multiple events occur during transformation and tumorigenesis by the transcapsidant virus populations and provide an explanation for the multiplicity of findings which have been reported with these virus populations.

MeSH Terms
Adenoviridae/immunology Animals Antigens Cell Line Cell Nucleus Cell Transformation, Neoplastic Cricetinae Fluorescent Antibody Technique Genetics, Microbial Haplorhini Hybridization, Genetic Immune Sera Inclusion Bodies, Viral Kidney Neoplasm Transplantation Neoplasms, Experimental/immunology Simian virus 40/immunology
Chemicals
Antigens Immune Sera
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Black P H
Berman L D
Dixon C B
References (35)
35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.4.5.694-703.1969
Published
1969-11-00
Pages
694-703
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC375927
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.4.5.694-703.1969 PMC Full Text View journal details
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