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PMID: 4316022 Published · ppublish English Journal Article

Characteristics of and relationship between C particles and intracisternal A particles in cloned cell strains.

Journal of virology ·Vol. 2 ·No. 3 ·1968-03-00 ·Pages 238-47

Hall WT, Hartley JW, Sanford KK

Abstract

Four murine tissue culture cell strains, which originated by cloning from one common cell of subcutaneous connective tissue origin, were examined for the presence of virus by electron microscopy and complement fixation techniques. The relative distribution of C particles and intracisternal A particles was determined. Thereafter, the characteristics of and relationship between A- and C-type particles were investigated. Cell extracts were passaged onto virus-free Swiss and C3Hf mouse embryo tissue cultures; CF and EM tests were again made to investigate the infective capacities of the various particle types. Although C particles were found in passage cultures exposed to extracts from the C particle-bearing strains, no A particles were found in any passage culture. These results indicate that the intracisternal A particle was neither infective nor developmentally associated with the C particle. A positive CF test was correlated with the presence of morphologically detectable C particles and was independent of the concentration of A particles.

MeSH Terms
Animals Antigens Clone Cells Complement Fixation Tests Connective Tissue/microbiology Connective Tissue Cells Culture Techniques Embryo, Mammalian Endoplasmic Reticulum/microbiology Leukemia Virus, Murine/immunology,isolation & purification Mice Microscopy, Electron Oncogenic Viruses/immunology
Chemicals
Antigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hall W T
Hartley J W
Sanford K K
References (33)
33 references, click to expand
  1. Alterations in morphology, arginase, and beta-glucuronidase within a clone of mouse tumor cells in vitro.
    J Natl Cancer Inst. 1961 May;26:1193-219 PMID: 13746236
  2. PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY.
    Stain Technol. 1964 Mar;39:111-4 PMID: 14127791
  3. The G (Gross) leukemia antigen.
    Cancer Res. 1965 Jul;25(6):813-9 PMID: 4284252
  4. Electron microscope study of a cell-free induced leukemia of the mouse: a preliminary report.
    J Biophys Biochem Cytol. 1958 Mar 25;4(2):151-6 PMID: 13525427
  5. Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):780-6 PMID: 5219685
  6. Studies with the electron microscope of virus-host relationships in Ehrlich ascites tumor cells. II. The localization and possible development of anopheles A virus within the endoplasmic reticulum of the host cell.
    J Exp Med. 1955 Oct 1;102(4):371-8 PMID: 13263479
  7. [Presence of particles of viral appearance in a transplantable mouse sarcoma induced by methylcholanthrene].
    Bull Assoc Fr Etud Cancer. 1960 Apr-Jun;47:291-307 PMID: 13707803
  8. Electron microscopy of tumor cells and tumor viruses; a review.
    Cancer Res. 1958 Jun;18(5):491-509 PMID: 13547041
  9. The electron microscopy of normal and malignant cells.
    Ann N Y Acad Sci. 1956 Mar 30;63(6):1117-40 PMID: 13314464
  10. Virus particles and murine leukemia virus complement-fixing antigen in neoplastic and nonneoplastic cell lines.
    Science. 1967 Apr 7;156(3771):85-8 PMID: 4289746
  11. [Presence of virus-like particles in tumor tissue of mice with spontaneous leukemia].
    C R Hebd Seances Acad Sci. 1958 Nov 17;247(20):1802-5 PMID: 13609052
  12. CHEMICALLY DEFINED MEDIA FOR CULTIVATION OF LONG-TERM CELL STRAINS FROM FOUR MAMMALIAN SPECIES.
    Exp Cell Res. 1964 Dec;36:439-74 PMID: 14242231
  13. The detection and study of tumor viruses with the electron microscope.
    Cancer Res. 1960 Jun;20:712-27 PMID: 13799753
  14. Complement fixation and tissue culture assays for mouse leukemia viruses.
    Proc Natl Acad Sci U S A. 1965 May;53(5):931-8 PMID: 4287967
  15. Morphology and development of some murine leukemia viruses.
    Natl Cancer Inst Monogr. 1966 Sep;22:107-38 PMID: 4288556
  16. Electron microscopy of plasma-cell tumors of the mouse. I. MPC-1 and X5563 tumors.
    J Biophys Biochem Cytol. 1961 Feb;9:353-68 PMID: 13733008
  17. An electron microscopic study of a series of murine lymphoid neoplasms.
    J Natl Cancer Inst. 1961 Oct;27:747-91 PMID: 13883254
  18. A comparative electron microscopic study on Ehrlich ascites tumor cells, Yoshida sarcoma cells, and human cancerous peritonitis ascites cells.
    Cancer Res. 1958 Nov;18(10):1167-70 PMID: 13596958
  19. An electron microscope study of the morphology and distribution of the intracytoplasmic virus-like particles of Ehrlich ascites tumor cells.
    J Biophys Biochem Cytol. 1957 Mar 25;3(2):161-70 PMID: 13438899
  20. Virus-like bodies in a transplantable mouse plasma cell tumour.
    Nature. 1958 Apr 26;181(4617):1213 PMID: 13541434
  21. Studies with the electron microscope of virus-host relationships in Ehrlich ascites tumor cells. I. The identification and structure of anopheles A virus.
    J Exp Med. 1955 Oct 1;102(4):361-70 PMID: 13263478
  22. Clonal analysis of variant cell lines transformed to malignant cells in tissue culture.
    J Natl Cancer Inst. 1959 Nov;23:1035-59 PMID: 14441477
  23. Some ultrastructural characteristics of a series of primary and transplanted plasma-cell tumors of the mouse.
    J Natl Cancer Inst. 1961 May;26:1221-67 PMID: 13719477
  24. Occurrence of bodies within endoplasmic reticulum of Ehrlich ascites tumor cells.
    Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):828-31 PMID: 13370541
  25. Persistent Theiler's virus in ependymoma tissue culture and the problem of virus-like bodies seen by electron microscopy.
    J Natl Cancer Inst. 1961 Oct;27:793-805 PMID: 14484769
  26. The development of variations in transplantability and morphology within a clone of mouse fibroblasts transformed to sarcoma-producing cells in vitro.
    J Natl Cancer Inst. 1954 Oct;15(2):215-37 PMID: 13233880
  27. Studies on the difference in sarcoma-producing capacity of two lines of mouse cells derived in vitro from one cell.
    J Natl Cancer Inst. 1958 Jan;20(1):121-45 PMID: 13502776
  28. Micromorphology of murine tumor viruses and of affected cells.
    Fed Proc. 1962 Nov-Dec;21:936-41 PMID: 14024779
  29. Electron microscopy of plasma-cell tumors of the mouse. II. Tissue cultures of the X5563 tumor.
    J Biophys Biochem Cytol. 1961 Feb;9:369-81 PMID: 13733007
  30. In vitro and in vivo observations on a murine C-type virus.
    J Virol. 1967 Dec;1(6):1238-54 PMID: 4316251
  31. Virus-like particles in established murine cell lines: electron-microscopic observations.
    Science. 1967 Mar 24;155(3769):1543-5 PMID: 4289822
  32. Virus-like particles in association with L strain cells.
    Cancer Res. 1961 Feb;21:193-7 PMID: 13719412
  33. Further electron microscope studies of a mouse leukemia induced by cell-free filtrates.
    J Biophys Biochem Cytol. 1960 Jul;7:747-52 PMID: 13814781
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.2.3.238-247.1968
Published
1968-03-00
Pages
238-47
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC375606
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.2.3.238-247.1968 PMC Full Text View journal details
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