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PMID: 4318090 Published · ppublish English Journal Article

Biophysical comparison of two canine adenoviruses.

Journal of virology ·Vol. 5 ·No. 4 ·1970-04-00 ·Pages 507-12

Marusyk RG, Norrby E, Lundqvist U

Abstract

The two canine adenoviruses, infectious canine hepatitis (ICH) virus and infectious canine laryngotracheitis (ICL) virus (also designated as Toronto A26/61 virus), were studied with respect to their morphology and the biological properties of their soluble components. The two viruses were found to be composed of soluble components similar to, and carrying biological activities corresponding to, those of the human adenoviruses after fractionation by rate zonal centrifugation and anion-exchange chromatography. The following soluble components were identified: hexons (carrying a common group-specific complement-fixing antigen), penton oligomers (complete soluble hemagglutinin), and penton and fiber monomers (incomplete soluble hemagglutinins). The latter were indicated by hemagglutination enhancement with selected antisera directed against human adenovirus soluble components. Elution characteristics of corresponding components of the two viruses in anion-exchange chromatography experiments were distinctly different. Electron microscopic examination of purified virions and soluble components revealed the fiber component of ICL virus to be 35 to 37 nm in length, and that of ICH virus to be 25 to 27 nm long.

MeSH Terms
Adenoviridae/immunology Antigens/analysis,isolation & purification Centrifugation, Density Gradient Centrifugation, Zonal Cesium Chlorides Chromatography, Ion Exchange Complement Fixation Tests Hemagglutination Inhibition Tests Hemagglutination Tests Hemagglutinins, Viral/analysis,isolation & purification Hepatovirus/immunology Immune Sera Microscopy, Electron Sucrose Trypsin/metabolism
Chemicals
Antigens Chlorides Hemagglutinins, Viral Immune Sera Cesium Sucrose Trypsin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Marusyk R G
Norrby E
Lundqvist U
References (20)
20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.5.4.507-512.1970
Published
1970-04-00
Pages
507-12
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376033
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.5.4.507-512.1970 PMC Full Text View journal details
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