Identification of proinsulin and C-peptide in human serum by a specific immunoassay.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 1 ·1970-09-00 ·Pages 148-55

Melani F, Rubenstein AH, Oyer PE, Steiner DF

Abstract

An immunoassay for the measurement of human proinsulin and C-peptide is described. By a combination of this system and an insulin immunoassay, proinsulin, insulin, and C-peptide were identified in acid-ethanol extracts of serum after gel filtration. In keeping with the results of biosynthetic studies, insulin and C-peptide were found to be present in equimolar amounts. The absolute values of serum proinsulin determined independently in the two assays corresponded closely.

MeSH Terms

Adenoma, Islet Cell/blood Amino Acid Sequence Animals Antigen-Antibody Reactions Cattle Chromatography, Gel Humans Immunoassay Insulin/biosynthesis Iodine Isotopes Methods Obesity/blood Peptides/blood Rabbits Species Specificity Swine

Chemicals

Insulin Iodine Isotopes Peptides

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Melani F

Rubenstein A H

Oyer P E

Steiner D F

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.1.148

Published

1970-09-00

Pages

148-55

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283181

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.1.148 PMC Full Text View journal details

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Identification of proinsulin and C-peptide in human serum by a specific immunoassay.

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