Structure and biological activity of deoxyribonucleic acid from Bacillus bacteriophage phi 105: effects of Escherichia coli exonucleases.

Journal of virology ·Vol. 7 ·No. 3 ·1971-03-00 ·Pages 359-71

Armentrout RW, Skoog L, Rutberg L

Abstract

The effects of Escherichia coli exonuclease I, exonuclease III, and deoxyribonucleic acid (DNA) polymerase on the biological activity of mature DNA from temperate Bacillus bacteriophage phi105 were investigated. Intact DNA loses infectivity rapidly upon exposure to exonuclease III. Although there is an overall decrease in marker rescue from exonuclease III-digested DNA, digestion preferentially affects markers at the end of the genetic map. This is taken to indicate a nonpermuted gene sequence in mature DNA. Incubation of mature DNA in the presence of exonuclease I or DNA polymerase has no effect on its biological activity. The possible structure of the ends of mature phi105 DNA is discussed. The rate of digestion of mature phi105 DNA by exonuclease III is only about 1/20 the rate of lambda DNA. Results of digestion of various DNA substrates by exonuclease III indicate that the enzyme distinguishes between different DNA terminal structures.

MeSH Terms

Bacillus subtilis Bacteriophages/metabolism Carbon Isotopes Centrifugation, Density Gradient Cesium Chlorides Chromosome Mapping Coliphages DNA Nucleotidyltransferases/metabolism DNA, Viral/isolation & purification,metabolism Escherichia coli/enzymology Nucleotides/metabolism Phosphoric Monoester Hydrolases/metabolism Sucrose Thymidine/metabolism Tritium

Chemicals

Carbon Isotopes Chlorides DNA, Viral Nucleotides Tritium Cesium Sucrose DNA Nucleotidyltransferases Phosphoric Monoester Hydrolases Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Armentrout R W

Skoog L

Rutberg L

References (26)

26 references, click to expand

COHESION OF DNA MOLECULES ISOLATED FROM PHAGE LAMBDA.
Proc Natl Acad Sci U S A. 1963 May;49(5):748-55 PMID: 16591099
Mapping of prophage and mature deoxyribonucleic acid from temperate Bacillus bacteriophage phi 105 by marker rescue.
J Virol. 1970 Dec;6(6):760-7 PMID: 5495510
Characterization of Temperate Bacillus Bacteriophage phi105.
J Virol. 1969 Sep;4(3):264-70 PMID: 16789103
Structure and base sequence in the cohesive ends of bacteriophage lambda DNA.
J Mol Biol. 1968 Aug 14;35(3):523-37 PMID: 4299833
Mechanism of transfection with deoxyribonucleic acid from the temperate Bacillus bacteriophage phi-105.
J Virol. 1969 Jul;4(1):50-7 PMID: 4980072
Changes in the structure and activity of lambda DNA in a superinfected immune bacterium.
J Mol Biol. 1965 Dec;14(2):399-417 PMID: 5326412
Enzymes in DNA metabolism.
Annu Rev Biochem. 1969;38:795-840 PMID: 4308606
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.
J Biol Chem. 1964 Jan;239:222-32 PMID: 14114848
Nucleotide sequence analysis of DNA. I. Partial sequence of the cohesive ends of bacteriophage lambda and 186 DNA.
J Mol Biol. 1970 Aug;51(3):501-21 PMID: 4321727
Influence of glucosylation of deoxyribonucleic acid on hydrolysis by deoxyribonucleases of Escherichia coli.
J Biol Chem. 1966 May 10;241(9):2084-92 PMID: 4287760
BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS.
J Bacteriol. 1965 Mar;89:782-90 PMID: 14273661
Mapping of a temperate bacteriophage active on Bacillus subtilis.
J Virol. 1969 Jan;3(1):38-44 PMID: 4977240
A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY.
J Biol Chem. 1964 Jan;239:242-50 PMID: 14114850
Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.
J Biol Chem. 1969 Jun 10;244(11):2996-3008 PMID: 4890762
Sedimentation rate as a measure of molecular weight of DNA.
Biophys J. 1963 Jul;3:309-21 PMID: 14016998
Characterization of infectious deoxyribonucleic acid from temperature Bacillus subtilis bacteriophage phi105.
J Virol. 1970 May;5(5):604-8 PMID: 4986850
Calibration of molecular weight scales for DNA.
J Mol Biol. 1969 Dec 14;46(2):313-28 PMID: 5360042
Low-frequency rescue of a genetic marker in deoxyribonucleic acid from Bacillus bacteriophage phi 105 by superinfecting bacteriophage.
J Virol. 1970 Dec;6(6):768-71 PMID: 5495511
Terminal repetition in non-permuted T3 and T7 bacteriophage DNA molecules.
J Mol Biol. 1967 Feb 14;23(3):365-76 PMID: 6032184
A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.
J Biol Chem. 1964 Jan;239:251-8 PMID: 14114851
The P22 bacteriophage DNA molecule. I. The mature form.
J Mol Biol. 1968 Oct 14;37(1):21-40 PMID: 4939038
The arrangement of information in DNA molecules.
J Gen Physiol. 1966 Jul;49(6):143-69 PMID: 5967428
Interpretation of sucrose gradient sedimentation pattern of deoxyribonucleic acid fragments resulting from random breaks.
J Virol. 1969 Jul;4(1):24-30 PMID: 5804949
The amino acid composition of T3 bacteriophage.
J Biol Chem. 1953 Nov;205(1):291-5 PMID: 13117907
Heat denaturation of P2 bacteriophage DNA: compositional heterogeneity.
J Mol Biol. 1969 Sep 28;44(3):533-49 PMID: 4899476
The genetics and physiology of bacteriophage T7.
Virology. 1969 Nov;39(3):562-74 PMID: 4902069

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.3.359-371.1971

Published

1971-03-00

Pages

359-71

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356126

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.3.359-371.1971 PMC Full Text View journal details

Analysis Services

Structure and biological activity of deoxyribonucleic acid from Bacillus bacteriophage phi 105: effects of Escherichia coli exonucleases.

Contact

WeChat Official Account