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PMID: 4324565 Published · ppublish English Journal Article

Composition of cellular membranes in the pancreas of the guinea pig. 3. Enzymatic activities.

The Journal of cell biology ·Vol. 49 ·No. 1 ·1971-04-00 ·Pages 150-8

Meldolesi J, Jamieson JD, Palade GE

Abstract

A COMPARATIVE STUDY OF THE ENZYMIC ACTIVITIES OF MEMBRANE FRACTIONS DERIVED FROM GUINEA PIG PANCREATIC HOMOGENATES HAS YIELDED THE FOLLOWING RESULTS: Rough microsomal membranes (derived from the rough ER) have the reductase activities of the two microsomal electron transport systems but lack enzyme activities of Golgi-type (TPPase) and plasmalemmal-type (5'-nucleotidase, beta-leucyl naphthylamidase, Mg-ATPase). Smooth microsomal membranes (derived primarily from the Golgi complex), zymogen granule membranes, and plasmalemmal fractions possess overlapping enzyme activities of plasmalemmal type, in different relative concentrations for each fraction. In addition, the smooth microsomal membranes exhibit TPPase and ADPase activity and share with rough microsomes the reductase activities of the two electron transport chains. Taken together with recent data on the lipid composition of the same fractions (2), these results indicate that the membranes of the pancreatic exocrine cell are chemically and functionally distinct, and hence do not mix with one another during the transport of secretory products.

MeSH Terms
Adenosine Triphosphatases/metabolism Aging Amidohydrolases/metabolism Animals Cell Membrane/enzymology Cold Temperature Cytochromes/metabolism Electron Transport Electron Transport Complex IV/metabolism Endoplasmic Reticulum/enzymology Enzyme Precursors/metabolism Esterases/metabolism Golgi Apparatus/enzymology Guinea Pigs Histocytochemistry Magnesium/metabolism Male Microsomes/enzymology NAD/metabolism Nucleotidases/metabolism Oxidoreductases/metabolism Pancreas/enzymology Phosphoric Monoester Hydrolases/metabolism
Chemicals
Cytochromes Enzyme Precursors NAD Oxidoreductases Electron Transport Complex IV Esterases Nucleotidases Phosphoric Monoester Hydrolases Amidohydrolases Adenosine Triphosphatases Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meldolesi J
Jamieson J D
Palade G E
References (29)
29 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.49.1.150
Published
1971-04-00
Pages
150-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108200
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.49.1.150 PMC Full Text View journal details
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