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PMID: 4326323 Published · ppublish English Journal Article

Pharmacological properties of centrally administered ouabain and their modification by other drugs.

British journal of pharmacology ·Vol. 42 ·No. 2 ·1971-06-00 ·Pages 242-53

Doggett NS, Spencer PS

Abstract

1. Ouabain given by intracerebroventricular injection to mice in small doses (0.1-0.4 mug) produced a dose related depression of central nervous activity, characterized by a reduction in spontaneous locomotor activity, hypothermia, catalepsy and ptosis, lowered body posture and lack of response to external stimuli. Doses above 0.4 mug were excitatory, convulsant and lethal.2. The depressant effects could be antagonized by (+)-amphetamine, desmethylimipramine, dibutyryl cyclic 3'5'-adenosine monophosphate and caffeine.3. The MAO inhibitor nialamide produced only a small antagonism of ouabain, resulting in a greater rate of recovery from the depressant effects of ouabain.4. The depressant effects were associated with a marked elevation of whole-brain dopamine levels with little change in noradrenaline or 5-hydroxytryptamine.5. The dopamine-beta-hydroxylase inhibitor sodium diethyldithiocarbamate, administered by intracerebroventricular injection, produced effects qualitatively similar to those seen after ouabain.6. An interference with central transmitter function is postulated as a possible mode of action of intracerebroventricularly injected ouabain.

MeSH Terms
Adenine Nucleotides/pharmacology Amphetamine/pharmacology Animals Body Temperature/drug effects Caffeine/pharmacology Central Nervous System/drug effects Cerebral Ventricles Depression, Chemical Desipramine/pharmacology Dopamine/metabolism Injections Male Mice Mixed Function Oxygenases/antagonists & inhibitors Motor Activity/drug effects Nialamide/pharmacology Norepinephrine/metabolism Ouabain/administration & dosage,antagonists & inhibitors,pharmacology Posture/drug effects Serotonin/metabolism Synaptic Transmission/drug effects Thiocarbamates/pharmacology
Chemicals
Adenine Nucleotides Thiocarbamates Serotonin Caffeine Ouabain Amphetamine Mixed Function Oxygenases Nialamide Desipramine Dopamine Norepinephrine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Doggett N S
Spencer P S
References (26)
26 references, click to expand
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1971.tb07105.x
Published
1971-06-00
Pages
242-53
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1667160
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1971.tb07105.x PMC Full Text View journal details
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