Purification of the Sabin strain of poliovirus type I through treatment with sarkozyl.

Journal of virology ·Vol. 7 ·No. 5 ·1971-05-00 ·Pages 687-9

Fiszman M, Bucchini D, Girard M

Abstract

Sodium dodecyl sulfate was found to aggregate virions from the Sabin Lsc2ab strain of poliovirus type 1. Aggregation was prevented by high ionic strength buffers. A procedure is described for the rapid purification of the virus through the use of sarkozyl.

MeSH Terms

Amino Acids Buffers Carbon Isotopes Centrifugation, Density Gradient Dactinomycin Detergents Edetic Acid Electrophoresis, Disc HeLa Cells Hydrochloric Acid Methods Poliovirus/isolation & purification Sodium Sodium Chloride Sucrose Sulfates Tritium Uridine Virus Cultivation

Chemicals

Amino Acids Buffers Carbon Isotopes Detergents Sulfates Tritium Dactinomycin Sodium Chloride Sucrose Edetic Acid Sodium Hydrochloric Acid Uridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Fiszman M

Bucchini D

Girard M

References (8)

8 references, click to expand

Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells.
Virology. 1968 Sep;36(1):48-54 PMID: 4299390
Molecular weight of poliovirus ribonucleic acid.
J Virol. 1969 Oct;4(4):475-9 PMID: 4309881
Inhibition of poliovirus RNA synthesis by supraoptimal temperatures.
J Gen Virol. 1970 Feb;6(2):293-304 PMID: 4314410
Evidence for ambiguity in the posttranslational cleavage of poliovirus proteins.
Virology. 1970 Jul;41(3):408-18 PMID: 4316554
Capsid proteins of Simian virus 40.
Biochem Biophys Res Commun. 1970 Jul 13;40(1):97-102 PMID: 4318589
A CYTOPLASMIC STRUCTURE INVOLVED IN THE SYNTHESIS AND ASSEMBLY OF POLIOVIRUS COMPONENTS.
J Mol Biol. 1964 Apr;8:541-55 PMID: 14153525
THE EXTRACTION OF RIBONUCLEIC ACID FROM POLIOVIRUS BY TREATMENT WITH SODIUM DODECYL SULFATE.
Virology. 1964 Mar;22:360-7 PMID: 14127833

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.5.687-689.1971

Published

1971-05-00

Pages

687-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356181

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.5.687-689.1971 PMC Full Text View journal details

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Purification of the Sabin strain of poliovirus type I through treatment with sarkozyl.

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