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PMID: 43286 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Bacterial aggregating activity in human saliva: simultaneous determination of free and bound cells.

Infection and immunity ·Vol. 26 ·No. 3 ·1979-12-00 ·Pages 1028-34

Golub EE, Thaler M, Davis C, Malamud D

Abstract

Two new assays for saliva-mediated aggregation of oral bacteria have been developed, based on the use of [3H]thymidine-labeled cells. One assay separates free cells from aggregated cells by centrifugation through sucrose, whereas the other utilizes membrane filters (8 micrometers, Nuclepore) to effect the separation. Comparison of these assays with the turbidity method reveals that they are faster (X20 to 40) and require 10 times less saliva and bacteria. The aggregation of Streptococcus sanguis M5, as determined with these assays, is complete in 5 min and is dose dependent on added cells and saliva. The reaction exhibits a temperature optimum of 42 degrees C with no reaction at 0 degrees C. If the pH is reduced to below 5, saliva-dependent aggregation is inhibited. The salivary factor(s) are heat labile, losing 100% of their activity after 100 degrees C, 10 min or 70 degrees C, 30 min.

MeSH Terms
Bacterial Physiological Phenomena Centrifugation Filtration Humans Hydrogen-Ion Concentration Saliva/analysis,microbiology Salivary Proteins and Peptides/analysis Streptococcus sanguis/physiology Temperature Thymidine Tritium
Chemicals
Salivary Proteins and Peptides Tritium Thymidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Golub E E
Thaler M
Davis C
Malamud D
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22 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.26.3.1028-1034.1979
Published
1979-12-00
Pages
1028-34
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414723
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.26.3.1028-1034.1979 PMC Full Text View journal details
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