Characterization of the genome of cytoplasmic polyhedrosis virus.

Journal of virology ·Vol. 7 ·No. 4 ·1971-04-00 ·Pages 434-7

Lewandowski LJ, Millward S

Abstract

It is possible to oxidize in situ the 3'-terminals of the double-stranded ribonucleic acid (RNA) contained in structurally intact cytoplasmic polyhedrosis virus. The number of 3'-terminals subsequently found by reduction with tritiated sodium borohydride was equivalent to the number observed in RNA first isolated from the virus and then oxidized and reduced. The viral RNA appears to occur in 10 distinct polynucleotide segments present in equimolar amounts.

MeSH Terms

Borohydrides Centrifugation, Density Gradient Cesium Chlorides Electrophoresis, Disc Insect Viruses/analysis Nucleosides/analysis Oxidation-Reduction Periodic Acid Phenols RNA, Viral/analysis,isolation & purification Sodium Sulfates Tritium

Chemicals

Borohydrides Chlorides Nucleosides Phenols RNA, Viral Sulfates Tritium Periodic Acid Cesium Sodium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lewandowski L J

Millward S

References (6)

6 references, click to expand

Characterization of a Ribonucleic Acid Polymerase Activity Associated with Purified Cytoplasmic Polyhedrosis Virus of the Silkworm Bombyx mori.
J Virol. 1969 Dec;4(6):857-65 PMID: 16789118
Segments of genome of viruses containing double-stranded ribonucleic acid.
J Mol Biol. 1970 Jul 28;51(2):247-53 PMID: 5485904
Double-stranded Ribonucleic Acid from Cytoplasmic Polyhedrosis Virus of the Silkworm.
J Virol. 1968 Oct;2(10):1211-22 PMID: 16789087
Structural studies on reovirus: discontinuities in the genome.
Proc Natl Acad Sci U S A. 1970 Feb;65(2):422-9 PMID: 4313198
Regulation of transcription of the Reovirus genome.
J Mol Biol. 1968 Aug 28;36(1):107-23 PMID: 5760535
Comparison of the ribonucleic Acid subunits of reovirus, cytoplasmic polyhedrosis virus, and wound tumor virus.
J Virol. 1969 Dec;4(6):851-6 PMID: 16789117

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.4.434-437.1971

Published

1971-04-00

Pages

434-7

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356141

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.4.434-437.1971 PMC Full Text View journal details

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Characterization of the genome of cytoplasmic polyhedrosis virus.

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