Analysis of the events leading to SV40-induced chromosome replication and mitosis in primary mouse kidney cell cultures.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 6 ·1971-06-00 ·Pages 1208-11

May E, May P, Weil R

Abstract

Abortive infection with simian virus 40 in confluent, "contact-inhibited", mouse kidney cell cultures was studied. The sequential events, in individual cells, are tentatively represented by the simplified scheme: (a) transcription of early virus-specific (messenger) RNA; (b) appearance of T-antigen; (c) [psychrosensitive event(s)]; (d) chromosome replication; (e) normal or abnormal mitosis. No evidence for the replication of viral progeny DNA was obtained. The sequence of the events (a)-(d) is analogous to that observed in contact-inhibited mouse kidney tissue culture cells during lytic infection with polyoma virus.

MeSH Terms

Animals Antigens, Viral/analysis Autoradiography Cells, Cultured/metabolism,microbiology Chromosomes Contact Inhibition DNA/analysis DNA Replication DNA, Viral/biosynthesis Fluorescent Antibody Technique Kidney/cytology Mice Mitosis Simian virus 40/immunology Thymidine/metabolism Time Factors Tritium

Chemicals

Antigens, Viral DNA, Viral Tritium DNA Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

May E

May P

Weil R

References (12)

12 references, click to expand

Polyoma virus-induced "complement-fixing antigen" in tumors and infected cells and detected by immunofluorescence.
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Acquisition of enzyme function by mouse kidney cells abortively infected with papovavirus SV40.
Proc Natl Acad Sci U S A. 1966 Aug;56(2):463-70 PMID: 4291009
Selective extraction of polyoma DNA from infected mouse cell cultures.
J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
[Study of the induction mechanism in Syrian hamster cells transformed by SV40 virus. I. Properties of a clonal cell line].
Int J Cancer. 1967 Mar 15;2(2):117-32 PMID: 4292331
Response of simian virus 40-transformed cell lines and cell hybrids to superinfection with simian virus 40 and its deoxyribonucleic acid.
J Virol. 1969 Oct;4(4):348-55 PMID: 4186276
Transplantable mouse tumor line induced by injection of SV40-transformed mouse kidney cells.
Int J Cancer. 1969 Jul 15;4(4):384-92 PMID: 4310402
A study on the transcription of the polyoma viral genome.
Proc Natl Acad Sci U S A. 1970 Jan;65(1):226-33 PMID: 4313194
Induction of cellular deoxyribonuleic acid synthesis by simian virus 40.
J Virol. 1967 Aug;1(4):738-46 PMID: 4316230
Polyoma "tumor antigen": an activator of chromosome replication?
Proc Natl Acad Sci U S A. 1970 Oct;67(2):1011-7 PMID: 4331714
Purification of polyoma virus.
Virology. 1963 Feb;19:158-68 PMID: 14001211
INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS.
Proc Natl Acad Sci U S A. 1964 Jul;52:53-9 PMID: 14192657
SV-40 INDUCED PROLIFERATION OF TISSUE CULTURE CELLS OF RABBIT, MOUSE, AND PORCINE ORIGIN.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.6.1208

Published

1971-06-00

Pages

1208-11

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389155

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.6.1208 PMC Full Text View journal details

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Analysis of the events leading to SV40-induced chromosome replication and mitosis in primary mouse kidney cell cultures.

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