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PMID: 4331298 Published · ppublish English Journal Article

Effects of puromycin on the structure of rat intestinal epithelial cells during fat absorption.

The Journal of cell biology ·Vol. 52 ·No. 1 ·1972-01-00 ·Pages 15-40

Friedman HI, Cardell RR

Abstract

This report provides information on the morphology of rat intestinal epithelial cells during fat absorption. In addition, the role of protein metabolism in this process has been evaluated by blocking its synthesis with puromycin and studying the fine structure of mucosal cells from rats at various times after fat intubation. The results indicate that SER-derived vesicles, containing fat droplets, migrate from the apical cytoplasm of the absorptive cell and fuse with saccules or vacuoles of the Golgi complex. Arguments are made that the Golgi complex is important in completing chylomicron formation and in providing appropriate enveloping membranes for the chylomicron. Such membranes may be necessary for Golgi vacuoles to fuse with the lateral cell membranes and release chylomicra. Puromycin treatment causes the absorptive cell to accumulate increased quantities of lipid that are devoid of membrane during fat absorption. In addition, puromycin-treated cells contain much less RER and Golgi membranes are strikingly decreased in number. In this paper we discuss the consequences of these abnormalities and suggest that continued protein synthesis by the RER is required in order to generate Golgi membranes. If such membranes are absent the cell's ability to discarge chylomicra is impaired and lipid accumulates.

MeSH Terms
Animals Basement Membrane Biological Transport, Active/drug effects Cell Membrane/metabolism Cell Nucleolus Cytoplasm/metabolism Desmosomes Endoplasmic Reticulum/drug effects Epithelial Cells Epithelium/drug effects Fasting Fats/metabolism Fatty Acids, Nonesterified/metabolism Golgi Apparatus/drug effects,metabolism Histocytochemistry Inclusion Bodies/analysis Intestinal Absorption/drug effects Intestinal Mucosa/cytology,drug effects Jejunum Lipids/analysis Male Methods Microscopy, Electron Mitochondria Protein Biosynthesis Proteins/antagonists & inhibitors Puromycin/pharmacology Rats Time Factors Triglycerides/biosynthesis
Chemicals
Fats Fatty Acids, Nonesterified Lipids Proteins Triglycerides Puromycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Friedman H I
Cardell R R
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48 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.52.1.15
Published
1972-01-00
Pages
15-40
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108684
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.52.1.15 PMC Full Text View journal details
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