Cytoplasmic DNA synthesis induced by RNA tumor viruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1844-7

Hatanaka M, Kakefuda T, Gilden RV, Callan EA

Abstract

Infection of mouse embryo cells with two strains of murine sarcoma virus and one strain of murine leukemia virus was followed rapidly by synthesis of DNA in the cytoplasm. Persistently infected cells have not shown such synthesis, and ultraviolet-irradiated virus did not induce DNA synthesis. This new DNA presumably represents an intermediate in the virus replication cycle specified by the virion DNA polymerase(s). Failure to observe cytoplasmic DNA synthesis in persistently infected cells suggests, in keeping with the results of inhibitor experiments, that the new "viral" DNA becomes associated with cellular DNA in some form of stable interaction.

MeSH Terms

Animals Autoradiography Cells, Cultured/metabolism Cytoplasm/metabolism DNA, Viral/analysis,biosynthesis Embryo, Mammalian Fibroblasts/metabolism,microbiology Gammaretrovirus/metabolism Mice Microscopy Microscopy, Electron Rauscher Virus/metabolism Thymidine/metabolism Time Factors Tritium Ultraviolet Rays Virus Replication

Chemicals

DNA, Viral Tritium Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hatanaka M

Kakefuda T

Gilden R V

Callan E A

References (8)

8 references, click to expand

Effect of infection with Moloney sarcoma and leukemia viruses on nucleic acid synthesis in mouse cell cultures.
J Natl Cancer Inst. 1970 Jan;44(1):107-16 PMID: 11515020
Initiation of deoxyribonucleic acid replication at the nuclear membrane in human cells.
J Mol Biol. 1968 Apr 14;33(1):225-9 PMID: 5646645
Mitochondrial DNA: advances, problems, and goals.
Science. 1968 Jul 4;165(3888):25-35 PMID: 4892070
RNA-dependent DNA polymerase in virions of RNA tumour viruses.
Nature. 1970 Jun 27;226(5252):1209-11 PMID: 4316300
RNA-dependent DNA polymerase in virions of Rous sarcoma virus.
Nature. 1970 Jun 27;226(5252):1211-3 PMID: 4316301
Light inactivation of focus formation by chicken embryo fibroblasts infected with avian sarcoma virus in the presence of 5-bromodeoxyuridine.
Nature. 1970 Nov 14;228(5272):622-4 PMID: 4319842
THE ROLE OF DEOXYRIBONUCLEIC ACID IN THE SYNTHESIS OF ROUS SARCOMA VIRUS.
Virology. 1964 Apr;22:462-8 PMID: 14166107
THE PARTICIPATION OF DNA IN ROUS SARCOMA VIRUS PRODUCTION.
Virology. 1964 Aug;23:486-94 PMID: 14204701

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.8.1844

Published

1971-08-00

Pages

1844-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389305

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.8.1844 PMC Full Text View journal details

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