Mechanism of action of bacitracin: complexation with metal ion and C 55 -isoprenyl pyrophosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 3223-7

Stone KJ, Strominger JL

Abstract

The inhibition by bacitracin of the enzymatic dephosphorylation of C(55)-isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a preincubation of bacitracin with a divalent cation and the lipid substrate, then its addition has little effect, indicating that bacitracin, metal ion, and C(55)-isoprenyl pyrophosphate form a complex. Various divalent cations can participate in complex formation, but monovalent cations are ineffective. A direct demonstration of the formation of a complex between the C(55)-isoprenyl pyrophosphate and bacitracin in the presence of metal ions was obtained. Molecular models that show one possible conformation for a complex between bacitracin and the C(55)-isoprenyl pyrophosphate, in which the metal ion acts as a bridge between the two compounds, are presented.

MeSH Terms

Bacitracin Cell Membrane Chelating Agents Chemical Phenomena Chemistry Chromatography, Gel Diphosphates Enterococcus faecalis Metals Micrococcus Models, Structural Phosphorus Isotopes Pyrophosphatases

Chemicals

Chelating Agents Diphosphates Metals Phosphorus Isotopes Bacitracin Pyrophosphatases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stone K J

Strominger J L

References (10)

10 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.12.3223

Published

1971-12-00

Pages

3223-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389626

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.12.3223 PMC Full Text View journal details

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Mechanism of action of bacitracin: complexation with metal ion and C 55 -isoprenyl pyrophosphate.

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