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PMID: 4332137 Published · ppublish English Journal Article

Regulation of protein synthesis in HeLa cells. 3. Inhibition during poliovirus infection.

Journal of virology ·Vol. 8 ·No. 5 ·1971-11-00 ·Pages 661-8

Leibowitz R, Penman S

Abstract

The mechanism of inhibition of host cell protein synthesis by poliovirus has been studied by examining the behavior of host polyribosomes and polyribosome-associated messenger ribonucleic acid (mRNA). Virus infection appears to result in a specific inhibition of the initiation of translation of host cell mRNA. The site of inhibition does not appear to be mRNA itself. Human cells respond to virus infection by producing a factor antagonistic to the virus inhibition which promotes the initiation of host mRNA translation. The production of the host factor is sensitive to actinomycin; however, the behavior of the host cell factor and that of host mRNA appear distinctly different.

MeSH Terms
Amino Acids/metabolism Carbon Isotopes Cell Fractionation Centrifugation, Density Gradient Cesium Chlorides Cycloheximide/pharmacology Dactinomycin/pharmacology Electrophoresis, Disc Genetic Code HeLa Cells/metabolism Humans Leucine/metabolism Molecular Weight Peptide Biosynthesis Poliovirus/pathogenicity Protein Biosynthesis Proteins/analysis RNA, Messenger/metabolism Ribosomes/metabolism Sucrose Tritium Uridine/metabolism
Chemicals
Amino Acids Carbon Isotopes Chlorides Proteins RNA, Messenger Tritium Dactinomycin Cesium Sucrose Cycloheximide Leucine Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leibowitz R
Penman S
References (21)
21 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.8.5.661-668.1971
Published
1971-11-00
Pages
661-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376244
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.8.5.661-668.1971 PMC Full Text View journal details
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