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PMID: 4332141 Published · ppublish English Journal Article

Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of primary infected Chinese hamster cells.

Journal of virology ·Vol. 8 ·No. 5 ·1971-11-00 ·Pages 708-15

Hirai K, Lehman J, Defendi V

Abstract

Simian virus 40 deoxyribonucleic acid (DNA) became associated in an alkaline-stable form with the DNA of Chinese hamster embryo cells at 15 to 20 hr post-infection, at the time when cell DNA synthesis and T antigen were induced. The integration process was not inhibited by d-arabinosyl cytosine and was only partially inhibited by cycloheximide.

MeSH Terms
Animals Antigens, Viral/metabolism Cell Line Cell Nucleus/analysis,metabolism Cell Transformation, Neoplastic Cells, Cultured/metabolism Centrifugation, Density Gradient Cricetinae Culture Techniques Cycloheximide/pharmacology Cytarabine/pharmacology DNA/biosynthesis,isolation & purification,metabolism DNA, Viral/metabolism Fluorescent Antibody Technique Leucine/metabolism Lysine/metabolism Nucleic Acid Hybridization RNA, Viral Simian virus 40/immunology,metabolism,pathogenicity Sucrose Surface-Active Agents Thymidine/metabolism Time Factors Tritium Valine/metabolism
Chemicals
Antigens, Viral DNA, Viral RNA, Viral Surface-Active Agents Cytarabine Tritium Sucrose DNA Cycloheximide Leucine Valine Lysine Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hirai K
Lehman J
Defendi V
References (18)
18 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.8.5.708-715.1971
Published
1971-11-00
Pages
708-15
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376251
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.8.5.708-715.1971 PMC Full Text View journal details
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