Teichoic acid hydrolase activity in soil bacteria (Bacillus subtilis-sporulation-phosphodiesterase-polyamines-concanavalin A).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 1 ·1972-01-00 ·Pages 233-7

Wise EM, Glickman RS, Teimer E

Abstract

Bacterial phosphodiesterases have been found that are capable of cleaving the backbone of teichoic acid. Such enzymes have not been reported previously. An aerobic, gram-negative, rod-shaped bacterium producing this activity was detected and isolated by autoradiography of soil suspensions growing on minimal medium containing (32)P-labeled Bacillus subtilis ATCC 6051 cell walls as the sole phosphorus source. Broken-cell preparations are capable of depolymerizing teichoic acids in media of low ionic strength at near-neutral pH values. An active teichoicase is also present in B. subtilis, ATCC 6051 (the Marburg strain), especially in sporulating cultures.

MeSH Terms

Autoradiography Bacillus subtilis/enzymology,metabolism Carbon Isotopes Cell Wall/enzymology,metabolism Chromatography, Paper Culture Media Glycosides/metabolism Hydrolases/metabolism Phosphoric Acids/metabolism Phosphoric Monoester Hydrolases/metabolism Phosphorus Isotopes Soil Teichoic Acids/metabolism

Chemicals

Carbon Isotopes Culture Media Glycosides Phosphoric Acids Phosphorus Isotopes Soil Teichoic Acids Hydrolases Phosphoric Monoester Hydrolases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wise E M

Glickman R S

Teimer E

References (26)

26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.1.233

Published

1972-01-00

Pages

233-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427582

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.1.233 PMC Full Text View journal details

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Teichoic acid hydrolase activity in soil bacteria (Bacillus subtilis-sporulation-phosphodiesterase-polyamines-concanavalin A).

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