Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis.

Journal of bacteriology ·Vol. 109 ·No. 1 ·1972-01-00 ·Pages 134-9

Stroobant P, Young IG, Gibson F

Abstract

The ubiquinone precursor 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone was isolated from two ubiquinone-deficient mutants of Escherichia coli and identified by nuclear magnetic resonance and mass spectrometry. The results of genetic analysis of the mutants indicate that each mutant carries a mutation in a gene designated ubiG which was located, by cotransduction with the nalA and glpT genes, at minute 42 on the E. coli chromosome.

MeSH Terms

Cell-Free System Chemical Phenomena Chemistry Chromatography, Thin Layer Chromosome Mapping Conjugation, Genetic Culture Media Drug Resistance, Microbial Escherichia coli/drug effects,growth & development,isolation & purification,metabolism Gels Genes Glucose/metabolism Magnetic Resonance Spectroscopy Mass Spectrometry Mutagens Mutation Nalidixic Acid/pharmacology Nitrosoguanidines Quinones/analysis,biosynthesis,isolation & purification Silicon Dioxide Spectrophotometry Succinates/metabolism Transduction, Genetic Ubiquinone/biosynthesis Ultraviolet Rays

Chemicals

Culture Media Gels Mutagens Nitrosoguanidines Quinones Succinates Ubiquinone Nalidixic Acid Silicon Dioxide Glucose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Stroobant P

Young I G

Gibson F

References (13)

13 references, click to expand

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Current linkage map of Escherichia coli.
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Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone.
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THE GENETIC MAP OF ESCHERICHIA COLI K-12.
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J Bacteriol. 1965 Mar;89:680-6 PMID: 14273645
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Biochim Biophys Acta. 1967 Jun 13;141(1):1-7 PMID: 4860970
New multiprenylquinones in the biosynthesis of ubiquinone.
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Mutant strains of Escherichia coli K-12 unable to form ubiquinone.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.109.1.134-139.1972

Published

1972-01-00

Pages

134-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247260

Subset

External Links

PubMed source DOI: 10.1128/jb.109.1.134-139.1972 PMC Full Text View journal details

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Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis.

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