Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 2 ·1972-02-00 ·Pages 393-7

Livingston DM, Scolnick EM, Parks WP, Todaro GT

Abstract

A solid phase immunoadsorbent specific for RNA-dependent DNA polymerase from murine and feline RNA tumor viruses has been prepared. The enzymes from murine and feline virus but not from avian virus bind to columns of this material. Bound enzymes can be eluted in active form. This method has permitted selective purification of viral enzyme from crude extracts of virus-transformed cells, since the immunoadsorbent has no affinity for cellular DNA polymerases.

MeSH Terms

Alpharetrovirus/enzymology Animals Avian Sarcoma Viruses/enzymology Cats Cell Line Cell Transformation, Neoplastic Chromatography, Affinity Clone Cells DNA Nucleotidyltransferases/isolation & purification Detergents Gammaretrovirus/enzymology Immune Sera/isolation & purification Immunoelectrophoresis Immunoglobulin G/isolation & purification Mice Protein Binding RNA Viruses/enzymology RNA, Viral/analysis Rauscher Virus/enzymology Sulfates

Chemicals

Detergents Immune Sera Immunoglobulin G RNA, Viral Sulfates DNA Nucleotidyltransferases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Livingston D M

Scolnick E M

Parks W P

Todaro G T

References (10)

10 references, click to expand

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Selective enzyme purification by affinity chromatography.
Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43 PMID: 4971842
Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells.
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A general method for the isolation of labelled peptides from affinity-labelled proteins.
Biochem Biophys Res Commun. 1970 Feb 20;38(4):825-30 PMID: 5462703
The activation of mutant beta-galactosidase by specific antibodies. Purification of eleven antibody activatable mutant proteins and their subunits on sepharose immunosorbents. Determination of the molecular weights by sedimentation analysis and acrylamide gel electrophoresis.
Eur J Biochem. 1970 Dec;17(2):267-72 PMID: 5500395
Antibody to the RNA-dependent DNA polymerase of mammalian C-type RNA tumor viruses.
Proc Natl Acad Sci U S A. 1971 May;68(5):920-4 PMID: 4325004
Separation of murine cellular and murine leukaemia virus DNA polymerases.
Nat New Biol. 1971 Jun 9;231(23):163-7 PMID: 5281193
A general method for the specific isolation of peptides containing modified residues, using insoluble antibody columns.
Biochemistry. 1971 Jul 20;10(15):2828-34 PMID: 5165492
Affinity chromatography.
Annu Rev Biochem. 1971;40:259-78 PMID: 4399260
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.2.393

Published

1972-02-00

Pages

393-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426465

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.2.393 PMC Full Text View journal details

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Affinity chromatography of RNA-dependent DNA polymerase from RNA tumor viruses on a solid phase immunoadsorbent.

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