Home LiteratureArticle Details
PMID: 4333989 Published · ppublish English Journal Article

Inhibition of release of dopamine- -hydroxylase and norepinephrine from sympathetic nerves by colchicine, vinblastine, or cytochalasin-B (hypogastric nerve stimulation-exocytosis-microtubules-microfilaments-guinea pig).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 2 ·1972-02-00 ·Pages 520-2

Thoa NB, Wooten GF, Axelrod J, Kopin IJ

Abstract

Stimulation of the hypogastric nerve to the guinea pig vas deferens in the presence of phenoxybenzamine produced an enhanced release of both dopamine-beta-hydroxylase (EC 1.14.2.1) and norepinephrine. Addition of colchicine, vinblastine, or cytochalasin-B to the incubation medium caused an almost complete inhibition of release of both the amine and the enzyme. Colchicine and cytochalasin-B produced a blocking effect but did not, however, modify the ratio of dopamine-beta-hydroxylase to norepinephrine recovered in the bath fluid. The findings indicate that norepinephrine and dopamine-beta-hydroxylase are released from sympathetic nerve terminals by exocytosis and that this process is dependent upon the integrity of both microtubules and microfilaments.

MeSH Terms
Animals Colchicine/pharmacology Dopamine beta-Hydroxylase/antagonists & inhibitors,metabolism Guinea Pigs Hypogastric Plexus/drug effects,metabolism Male Microtubules/drug effects Mixed Function Oxygenases/metabolism Mycotoxins/pharmacology Neural Inhibition Norepinephrine/antagonists & inhibitors,metabolism Phenoxybenzamine/pharmacology Synaptic Transmission/drug effects Vas Deferens/drug effects,innervation Vinblastine/pharmacology
Chemicals
Mycotoxins Phenoxybenzamine Vinblastine Mixed Function Oxygenases Dopamine beta-Hydroxylase Colchicine Norepinephrine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Thoa N B
Wooten G F
Axelrod J
Kopin I J
References (18)
18 references, click to expand
  1. Effects of cytochalasins on mammalian cells.
    Nature. 1967 Jan 21;213(5073):261-4 PMID: 6067685
  2. Electron microscope study of the excretion of cathecol-containing droplets in the adrenal medulla.
    Exp Cell Res. 1957 Jun;12(3):568-74 PMID: 13437983
  3. Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells.
    J Cell Biol. 1968 Jul;38(1):224-9 PMID: 5691976
  4. New hypothesis of insulin secretion.
    Nature. 1968 Sep 14;219(5159):1177-9 PMID: 4877537
  5. Histamine release from rat peritoneal mast cells: inhibition by colchicine and potentiation by deuterium oxide.
    J Pharmacol Exp Ther. 1968 Nov;164(1):158-65 PMID: 4177465
  6. Effect of colchicine on transport of amine storage granules in sympathetic nerves of rat.
    Eur J Pharmacol. 1968 Dec;5(1):111-3 PMID: 5718512
  7. Properties of colchicine binding protein from chick embryo brain. Interactions with vinca alkaloids and podophyllotoxin.
    Biochemistry. 1970 Dec 8;9(25):4999-5007 PMID: 5480163
  8. Possible role of microtubules in thyroid secretion.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1901-8 PMID: 4321351
  9. Microfilaments in cellular and developmental processes.
    Science. 1971 Jan 15;171(3967):135-43 PMID: 5538822
  10. Studies on the translocation of noradrenaline-containing vesicles in post-ganglionic sympathetic neurones in vitro. Inhibition of movement by colchicine and vinblastine and evidence for the involvement of axonal microtubules.
    J Physiol. 1971 Aug;216(3):625-39 PMID: 4105241
  11. A possible role of microtubules in catecholamine release from the adrenal medulla: effect of colchicine, vinca alkaloids and deuterium oxide.
    J Pharmacol Exp Ther. 1971 Apr;177(1):102-8 PMID: 5566754
  12. Morphological aspects of exocytosin in the adrenal medulla.
    Philos Trans R Soc Lond B Biol Sci. 1971 Jun 17;261(839):291-2 PMID: 4399621
  13. Fine structure of noradrenaline storage vesicles in nerve terminals of the rat vas deferens.
    Philos Trans R Soc Lond B Biol Sci. 1971 Jun 17;261(839):319-23 PMID: 4108421
  14. Enhanced release of dopamine- -hydroxylase from sympathetic nerves by calcium and phenoxybenzamine and its reversal by prostaglandins.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2227-30 PMID: 5289380
  15. Proportional release of norepinephrine and dopamine- -hydroxylase from sympathetic nerves.
    Science. 1971 Dec 24;174(4016):1349-51 PMID: 5135722
  16. Cytochalasin B inhibits thyroid secretion.
    Biochem Biophys Res Commun. 1971 Jul 16;44(2):422-5 PMID: 4334139
  17. PROPERTIES OF NOREPINEPHRINE STORAGE PARTICLES OF THE RAT HEART.
    J Pharmacol Exp Ther. 1963 Dec;142:299-305 PMID: 14098325
  18. Isolation of a protein subunit from microtubules.
    J Cell Biol. 1967 Aug;34(2):549-54 PMID: 6035644
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.2.520
Published
1972-02-00
Pages
520-2
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426493
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.2.520 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2