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PMID: 4335066 Published · ppublish English Journal Article

Expression of differentiated functions in hepatoma cell hybrids: induction of mouse albumin production in rat hepatoma-mouse fibroblast hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 3 ·1972-03-00 ·Pages 571-5

Peterson JA, Weiss MC

Abstract

The synthesis of serum albumin has been studied in hybrids between well-differentiated rat hepatoma cells, which synthesize serum albumin, and mouse fibroblasts (3T3) that do not synthesize albumin. By immunodiffusion techniques with noncrossreacting antisera, the production of both rat and mouse albumin by the hybrids has been examined. Karyologically identified hybrids were produced between 3T3 cells and cells of a 1s hepatoma (Fu5) clone, and of a 2s hepatoma (2s Fu5-5cl.lE) clone. Each of the 3T3 x Fu5 hybrids produces only rat albumin. Among five 3T3 x 2s Fu5-5cl.lE hybrid clones isolated, one produces both rat and mouse albumin, two produce only mouse albumin, and two do not produce rat or mouse albumin.

MeSH Terms
Animals Carcinoma, Hepatocellular/metabolism Clone Cells Fibroblasts/metabolism Hybrid Cells/metabolism Immunodiffusion Karyotyping Liver Neoplasms Mice Neoplasms, Experimental/metabolism Rats Serum Albumin/analysis,biosynthesis
Chemicals
Serum Albumin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peterson J A
Weiss M C
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.3.571
Published
1972-03-00
Pages
571-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426509
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.3.571 PMC Full Text View journal details
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