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PMID: 4335658 Published · ppublish English Journal Article

Identification and genetic characterization of mutants of bacteriophage T4 defective in the ability to induce exonuclease A.

Journal of virology ·Vol. 9 ·No. 3 ·1972-03-00 ·Pages 399-407

Warner HR, Snustad DP, Koerner JF, Childs JD

Abstract

A mutant of bacteriophage T4 unable to induce exonuclease A has been isolated. The mutation responsible for this defect maps between genes 39 and 56, in a region of the chromosome devoid of other known markers. Four deletion mutants lacking part of the genome located between genes 39 and 56 also fail to induce exonuclease A. The ability of all of these mutants to replicate suggests that exonuclease A is not essential for replication of phage T4.

MeSH Terms
Cell-Free System Chromatography, DEAE-Cellulose Chromosome Mapping Coliphages/drug effects,growth & development,isolation & purification Cytosine Nucleotides DNA, Bacterial/metabolism Defective Viruses Deoxyribonucleases/biosynthesis,metabolism Enzyme Induction Escherichia coli/enzymology,metabolism Genetics, Microbial Hydroxyurea/pharmacology Methyltransferases/metabolism Mutation Nucleic Acid Denaturation Phosphoric Monoester Hydrolases/metabolism Recombination, Genetic Tetrahydrofolate Dehydrogenase/metabolism Virus Replication
Chemicals
Cytosine Nucleotides DNA, Bacterial Tetrahydrofolate Dehydrogenase Methyltransferases Deoxyribonucleases Phosphoric Monoester Hydrolases Hydroxyurea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Warner H R
Snustad D P
Koerner J F
Childs J D
References (25)
25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.9.3.399-407.1972
Published
1972-03-00
Pages
399-407
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356312
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.9.3.399-407.1972 PMC Full Text View journal details
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