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PMID: 4336123 Published · ppublish English Journal Article

Granulation tissue as a contractile organ. A study of structure and function.

The Journal of experimental medicine ·Vol. 135 ·No. 4 ·1972-04-01 ·Pages 719-34

Gabbiani G, Hirschel BJ, Ryan GB, Statkov PR, Majno G

Abstract

CONTRACTING GRANULATION TISSUES CONTAIN FIBROBLASTS THAT DEVELOP CHARACTERISTICS TYPICAL OF SMOOTH MUSCLE: (a) They contain an extensive cytoplasmic fibrillar system. (b) They show immunofluorescent labeling of their cytoplasm with human anti-smooth muscle serum. (c) The nuclei show complicated folds and indentations, indicative of cellular contraction. (d) There are cell-to-cell and cell-to-stroma attachments. (e) It is possible to extract similar quantities of actomyosin (having the same adenosine triphosphatase activity) from granulation tissue and from pregnant rat uterus. (f) Strips of granulation tissue, when tested pharmacologically in vitro, behave similarly to smooth muscle. All these data support the view that, under certain conditions, fibroblasts can differentiate into a cell type structurally and functionally similar to smooth muscle and that this cell, the "myo-fibroblast," plays an important role in connective tissue contraction.

MeSH Terms
Actomyosin/metabolism Adenosine Triphosphatases/metabolism Animals Autonomic Agents/pharmacology Cell Differentiation Desmosomes Endoplasmic Reticulum Fibroblasts Fluorescent Antibody Technique Granulation Tissue/cytology,drug effects,physiology Inclusion Bodies Male Microscopy, Electron Mitochondria Muscle Contraction Muscle, Smooth/cytology Myofibrils Rats Wound Healing
Chemicals
Autonomic Agents Actomyosin Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gabbiani G
Hirschel B J
Ryan G B
Statkov P R
Majno G
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25 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.135.4.719
Published
1972-04-01
Pages
719-34
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139162
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.135.4.719 PMC Full Text View journal details
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