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PMID: 4337705 Published · ppublish English Journal Article

Manganous ion as a spin label in studies of mitochondrial uptake of manganese.

Biophysical journal ·Vol. 12 ·No. 6 ·1972-06-00 ·Pages 625-35

Gunter TE, Puskin JS

Abstract

Manganous ion (Mn(2+)) has been used as a spin label for studies of divalent cation uptake by rat liver mitochondria. Spin exchange, observed in the electron paramagnetic resonance (EPR) spectrum of a fraction of the transported Mn(2+), shows that this fraction is bound in regions of high local concentration within the mitochondria. The average separation of manganese ions in that fraction is estimated to be 4.0 +/-0.6 A at the time of greatest concentration.

MeSH Terms
Animals Biological Transport, Active Edetic Acid Electron Spin Resonance Spectroscopy Manganese/metabolism Mitochondria, Liver/metabolism Rats
Chemicals
Manganese Edetic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gunter T E
Puskin J S
References (12)
12 references, click to expand
  1. A mechanism for the reactions of calcium with mitochondria.
    Proc Natl Acad Sci U S A. 1965 May;53(5):1069-76 PMID: 5222550
  2. [Structure and polymorphism of lipids: x-ray diffraction study of the system formed from beef heart mitochondria lipids and water].
    J Mol Biol. 1967 Jul 28;27(2):303-22 PMID: 6058223
  3. The binding of calcium at lipid-water interfaces.
    Eur J Biochem. 1967 Mar;1(1):61-9 PMID: 4293829
  4. Interactions of La3+ and local anesthetic drugs with mitochondrial Ca++ and Mn++ uptake.
    Arch Biochem Biophys. 1968 Feb;123(2):286-93 PMID: 4230613
  5. High affinity and low affinity binding of Ca++ by rat liver mitochondria.
    J Biol Chem. 1969 Feb 25;244(4):584-93 PMID: 5768858
  6. Electron paramagnetic resonance spectra of manganese (II)-protein complexes. Manganese (II)-concanavalin A.
    J Biol Chem. 1970 Feb 10;245(3):662-4 PMID: 4312870
  7. Mitochondria and calcium ion transport.
    Biochem J. 1970 Sep;119(2):129-38 PMID: 4922961
  8. The interaction of La 3+ with mitochondria in relation to respiration-coupled Ca 2+ transport.
    Arch Biochem Biophys. 1971 Apr;143(2):506-15 PMID: 5558134
  9. Isolation of a calcium-sequestering protein from sarcoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1231-5 PMID: 4256614
  10. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  11. Absolute yield measurements of radiation-produced radicals by electron spin resonance.
    Phys Med Biol. 1962 Apr;6:599-604 PMID: 14457678
  12. [Electron spin resonances in irradiated bacteriophages and their components. I].
    Strahlentherapie. 1963 Feb;120:161-90 PMID: 14003682
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(72)86108-3
Published
1972-06-00
Pages
625-35
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1484157
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(72)86108-3 PMC Full Text View journal details
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