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PMID: 4337848 Published · ppublish English Journal Article

Yeast membrane vesicles: isolation and general characteristics.

Journal of bacteriology ·Vol. 110 ·No. 3 ·1972-06-00 ·Pages 1190-205

Christensen MS, Cirillo VP

Abstract

Yeast membrane vesicles are formed when packed yeast are ground manually in a porcelain mortar and pestle with glass beads (0.2 mm diameter). These vesicles can be separated from the other components of the grinding mixture by a combination of centrifugation steps and elution from a column of the same glass beads (0.2 mm diameter). Isolated vesicles are osmotically sensitive, contain cytoplasmic components, and have energy-independent transport function. They are unable to metabolize glucose, but have respiratory function which is thought to be associated with intravesicular mitochondria. Invertase and oligomycin-insensitive adenosine triphosphatase are present in lysed vesicle preparations, and the appropriateness of these enzyme activities as membrane markers is discussed.

MeSH Terms
Adenosine Triphosphatases/antagonists & inhibitors Amino Acids/metabolism Cell Fractionation Cell Membrane/analysis,drug effects,enzymology,metabolism Electron Transport Complex IV/analysis Galactose/metabolism Glucose/metabolism Inclusion Bodies/analysis,drug effects,enzymology,metabolism Microscopy, Phase-Contrast Mitochondria/metabolism Oligomycins/pharmacology Osmotic Fragility Oxygen Consumption Potassium Chloride/pharmacology Saccharomyces/analysis,cytology,drug effects,enzymology,metabolism Sucrase/analysis
Chemicals
Amino Acids Oligomycins Potassium Chloride Electron Transport Complex IV Sucrase Adenosine Triphosphatases Glucose Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Christensen M S
Cirillo V P
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.110.3.1190-1205.1972
Published
1972-06-00
Pages
1190-205
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247543
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.110.3.1190-1205.1972 PMC Full Text View journal details
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