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PMID: 4340158 Published · ppublish English Journal Article

Identification of lactate dehydrogenase isoenzymes by rapid kinetics.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 7 ·1972-07-00 ·Pages 1761-5

Bishop MJ, Everse J, Kaplan NO

Abstract

Stopped-flow kinetics indicate that human and chicken heart-type(4) lactate dehydrogenases (LDH) become inhibited as DPNH is oxidized in the presence of high concentrations of pyruvate. This inhibition is much less marked with the human and chicken muscle-type(4) enzymes. The initial rates and the difference in inhibition between the two types of enzyme have made it possible to determine the amount of, as well as the ratio between, the two types of LDH that are present in a given sample by a single kinetic assay. The stopped-flow kinetic method has been used to analyze amounts of LDH isoenzyme in different tissues, as well as in serum.

MeSH Terms
Acetates Animals Chickens Hepatitis/enzymology Humans Isoenzymes Kidney/enzymology Kinetics L-Lactate Dehydrogenase/analysis,antagonists & inhibitors,blood Liver/enzymology Lung/enzymology Macromolecular Substances Mathematics Methods Muscles/enzymology Myocardium/enzymology NAD Pyridines Pyruvates Species Specificity Spleen/enzymology Temperature
Chemicals
Acetates Isoenzymes Macromolecular Substances Pyridines Pyruvates NAD L-Lactate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bishop M J
Everse J
Kaplan N O
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.7.1761
Published
1972-07-00
Pages
1761-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426797
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.7.1761 PMC Full Text View journal details
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